兔MSCs对韧带移植物骨道内愈合影响的光镜观察  

The morphology of effects of MSCs for healing of the graft of ligament in the bone tunnel

作　　者：刘靖[1] 徐斌[1] 徐洪港[1] 王瑞[1] 涂俊[1] 

机构地区：[1]安徽医科大学第一附属医院骨科四病区,合肥230022

出　　处：《安徽医科大学学报》2014年第8期1076-1079,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1208085MH157)

摘　　要：目的探讨兔自体外周血间充质干细胞(MSCs)对韧带移植物在骨道内愈合的影响。方法 8只新西兰白兔随机均分为实验组和对照组。粒细胞刺激因子动员各组兔后,抽取外周血分离、纯化培养干细胞。实验组移植入MSCs和生物蛋白胶混合物;对照组移植入生物蛋白胶。分别于2、4、8、12周4个时间点每组各处死1只兔行形态学观察,术后4个时间点对两组标本进行高倍镜下成纤维细胞计数,采用单向方差分析及SNK法检验,比较韧带重建术后移植肌腱与骨道愈合情况。结果术后8周实验组移植肌腱与骨道界面连接紧密,出现Sharpey纤维及软骨移行带,术后12周对照组出现Sharpey纤维,但连接组织中胶原排列紊乱。实验组与对照组在4个时间点高倍镜下成纤维细胞计数比较差异有统计学意义(P<0.05)。结论 MSCs能促进韧带重建术后移植肌腱在骨道内愈合。Objective To investigate whether autogenous peripheral blood mesenchymal stem cells gel could promote the healing of the graft of ligament in the bone tunnel.Methods 8 New Zealand white rabbits were randomly divided into experimental and control group.After mobilizeing with Granulocyte colony-stimulating,autogenous peripheral blood mesenchymal stem cells were isolated and cultured in vitro.For the experimental group,autogenous peripheral blood mesenchymal stem cells gel was injected into the the femur Road tip clearance of the ACL reconstruction model ; for the control group,only fibrin gluewas were injected into.Respectively,in 2nd week,4th week,8th week,12th week,one rabbit of each group was sacrificed for morphology and the number of fibroblasts was counted at tendon-bone interface with HE staining under high magnification microscope to investigate whether autogenous peripheral blood mesenchymal stem cells gel could promote the healing of tendon-bone interface after ligament reconstruction.Results For the experimental group,in 8th week,tendon-bone interface connection was tight,the interfacial region showed a large orderly arrangement of Sharpey fibers and chondrocyte-like cells,for the control group,in 12th week,Sharpey fibers could be found,but the collagen fiber was disorder.The number of fibroblasts was counted at tendon-bone interface,we adopted one-way ANOVA and SNK test to analyze the number of fibroblasts,the difference between experimental and control group was considered significant（P ＜ 0.05）.Conclusion Autogenous peripheral blood mesenchymal stem cells gel can promote the healing of tendon-bone interface after ligament reconstruction.

关 键 词：间充质干细胞 膝关节 韧带 移植物 愈合 

分 类 号：R686[医药卫生—骨科学]