尼卡地平对快速上浮脱险致减压病动物肺组织影响的研究  

Effect of nicardipine on lung injury in a fast floating escape induced decompression sickness animals

作　　者：攸璞[1] 方以群[1] 包晓辰[1] 李丹[2] 王海涛[1] 马骏[1] 王芳芳[1] 张师[1] 

机构地区：[1]海军医学研究所潜水医学研究室,上海200433 [2]海军总医院,北京100048

出　　处：《军事医学》2014年第7期488-489,492,共3页Military Medical Sciences

基　　金：总后勤部资助项目(12A106);海军后勤部资助项目(11K102)

摘　　要：目的研究尼卡地平对快速上浮脱险致减压病动物模型肺组织损伤的影响。方法雄性SD大鼠60只,随机分为3组,每组20只。实验组于进舱前0.5 h给予尼卡地平50 mg/kg灌胃,对照组于进舱前0.5 h给予相同体积的生理盐水灌胃,空白对照组仅放于加压舱内相同时间不做加压处理。空气以2t/7指数速率加压至1.5 MPa,停留4 min后匀速减压至常压出舱。出舱后0.5 h观察大鼠存活率、肺组织病理,通过ELISA检测IL1-β和TNF-α表达量,并通过Western印迹检测肺组织半胱天冬蛋白酶(caspase)3表达的改变。结果对照组发病率和死亡率分别为80%和50%,实验组发病率和死亡率分别为100%和80%。两组存活动物的肺组织病理均可见肺泡、肺间质出血,肺间质增宽。实验组TNF-α较正常组有明显升高,而IL1-β未见明显改变。尼卡地平处理后前半胱天冬蛋白酶(pro-caspase)3无显著改变,但裂解半胱天冬蛋白酶(cleaved-caspase)3明显增高。结论减压前使用尼卡地平对快速上浮脱险致减压病造成的肺损伤有促进作用,并促使了炎症加重和细胞凋亡增加。Objective To study the effect of nicardipine on fast floating escape induced lung injury in animal models with decompression sickness.Methods Sixty male SD rats were randomly and evenly divided into three groups：blank control,control and nicardipine groups.The nicardipine group was given nicardipine 50 mg/kg orally 0.5 h before entrance.In the control group,rats were given an equal volume of saline 0.5 h before entrance.The blank control group only stayed in the vehicle without any pressurized procedure.The air was pressurized at the 2t/7exponential rate to 1.5 Mpa which was maintained for 4 min,and then uniformly decompressed to atmospheric pressure.The extravehicular survival and lung pathology were observed in rats after 0.5 h,IL1-β and TNF-α expression levels were detected by ELISA,and the Caspase 3 expression in lung tissue was detected by Western blot.Results The incidence and mortality rate were 80% and50% respectively in control group,and 100% and 80% in the experimental group.The surviving animals in the two groups suffered from alveolar and interstitial lung hemorrhage,with widened interstitial lung.IL1-β in the experimental group was significantly higher than in the normal control group,while TNF-α had no significant change.After nicardipine treatment pro-caspase 3 did not change significantly,but cleaved-caspase 3 increased significantly.Conclusion Nicardipine can aggravate lung injury caused by fast floating escape-induced decompression sickness if used before decompression.

关 键 词：快速上浮脱险 减压病 尼卡地平 炎症因子 

分 类 号：R845.2[医药卫生—航空、航天与航海医学]