模拟400m氦氧饱和潜水对大鼠肺氧化与抗氧化系统的影响  

Effect of simulated 400 m He-O_2 saturation diving on oxidative-antioxidative system in lungs of rats

作　　者：杨涛[1] 黄志强[1] 李慈[1] 何佳[1] 陈海庭[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《军事医学》2014年第7期493-494,498,共3页Military Medical Sciences

基　　金：国家科技重大专项资助项目(2011ZX05027-005);全军后勤科研项目(AHJ08J003;AHJ11J004)

摘　　要：目的探讨模拟400 m氦氧饱和潜水对大鼠肺氧化与抗氧化系统的影响。方法将SD大鼠在4.1 MPa(绝对压力)氦氧高气压环境暴露24 h,以常压空气环境作为对照。检测肺组织病理学,测定肺组织丙二醛(MDA)、8-羟基脱氧鸟苷(8-OHdG)、还原型谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)与谷胱甘肽过氧化物酶(GSH-Px)活力。结果氦氧暴露大鼠肺组织病理学检查未见异常;肺组织匀浆中MDA、8-OHdG含量与SOD活力无明显变化,GSH-Px活力及GSH含量显著降低(P<0.05)。结论 4.1 MPa氦氧暴露24 h使大鼠肺处于氧化应激状态,抗氧化能力降低,但未造成明显的氧化损伤。Objective To investigate the effect of simulated 400 m He-O2 saturation diving on the pulmonary oxidativeantioxidative system in rats.Methods SD rats were exposed to 4.1 MPa He-O2 mixture for 24 h in an animal chamber.Normal air pressure was used in the control group.The histopathology,contents of malondialdehyde（MDA）,8-hydroxy-2＇-deoxyguanosine（8-OHdG）,and glutathione（GSH） and activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） in pulmonary tissues were determined.Results No difference was found during pathological examination or in MDA,8-OHdG and SOD activity.GSH and GSH-Px activity were decreased significantly（P〈0.05） after hyperbaric exposure.Conclusion Exposure to 4.1 MPa He-O2 for 24 h may promote oxidative stress and reduce antioxidative capacity rather than cause obvious oxidative damage in rat lungs.

关 键 词：氦氧饱和潜水 氧化应激 大鼠 抗氧化防御能力 

分 类 号：R847[医药卫生—航空、航天与航海医学]