消栓通络有效成分组对氧糖剥夺损伤原代培养神经元的保护作用  

Neuroprotective effects of the effective components group of Xiaoshuantongluo against oxygen-glucose deprivation in primary cultured rat cortical neurons

作　　者：谢欣梅[1] 庞晓斌[1] 赵艳[2] 王保全[3] 陈若芸 杜冠华 

机构地区：[1]河南大学药物研究所,河南开封475004 [2]青岛市市立医院,山东青岛266011 [3]河南大学淮河临床学院,河南开封475001 [4]中国医学科学院、北京协和医学院药物研究所国家药物筛选中心,北京100050

出　　处：《药学学报》2014年第8期1130-1135,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81273652)

摘　　要：研究消栓通络有效成分组(XECG)对原代培养皮质神经元氧糖剥夺(OGD)损伤的保护作用,并探讨其可能的作用机制。采用3日龄SD大鼠皮质制备原代培养神经元;将细胞随机分为正常对照组、OGD模型组和XECG组(1、3及10 mg·L-1);四甲基偶氮唑盐(MTT)法测定细胞存活率;酶标法试剂盒测定乳酸脱氢酶(LDH)漏出量;Hoechst染色检测细胞凋亡;RT-PCR检测JAK2、STAT3 mRNA表达变化;Western blotting检测Bcl-2、Bax、p-JAK2、p-STAT3蛋白表达变化。结果显示,XECG能明显改善OGD对神经元的损伤,提高细胞存活率,减少LDH的释放量,抑制缺氧缺糖引起的细胞凋亡;能提高缺糖缺氧神经元Bcl-2/Bax比值,增加JAK2、STAT3基因和蛋白的表达。以上结果表明,XECG对OGD损伤的原代培养神经元具有明显保护作用,其机制可能与激活JAK2/STAT3通路、影响凋亡相关基因Bcl-2及Bax的表达有关。This study is to investigate the effect of the effective components group of Xiaoshuantongluo （XECG） on neuronal injury induced by oxygen-glucose deprivation （OGD） in primary cortical cultures isolated from SD rat cortex at day 3 and the possible mechanism. Cells were divided into control group, OGD model group and XECG group （1, 3 and 10 mg·L^-1）. The cell viability was assessed with MTT assay and the LDH release rate was measured by enzyme label kit. The cell apoptosis was analyzed using Hoechst staining. RT-PCR was applied to detect the mRNA levels of JAK2 and STAT3. Western blotting was used to detect the expressions of Bcl-2, Bax, p-JAK2 and p-STAT3 proteins. Results showed that XECG resulted in an obvious resistance to oxygen-glucose deprivation-induced cell apoptosis and decrement of cell viability, decrease the cell LDH release rate. XECG could adjust the expression of Bcl-2 and Bax proteins and increase Bcl-2/Bax ratio, up-regulate the expression of p-JAK2 and p-STAT3. In conclusion, XECG could protect against the neuronal injury cells exposed to OGD, which may be relevant to the promotion of JAK2/STAT3 signaling pathway, andimpact the expression of Bax and Bcl-2.

关 键 词：消栓通络有效成分组(XECG) 原代培养神经元 糖氧剥夺 JAK2 STAT3信号通路 

分 类 号：R963[医药卫生—微生物与生化药学]