Local conformation transitions of linear DNA induced by cisplatin  

作　　者：Chao Ji Lingyun Zhang Shuoxing Dou Pengye Wang 

机构地区：[1]Key Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics,Chinese Academy of Sciences

出　　处：《Chinese Science Bulletin》2014年第25期3085-3089,共5页

基　　金：supported by the National Natural Science Foundation of China(11274374);the National BasicResearch Program of China(2009CB930704)

摘　　要：Cisplatin is the most successful anti-tumor drug,and its pharmacological property is generally considered to derive from the modification of DNA molecules.Structural modifications of short DNA induced by cisplatin have already been investigated.However,the conformation transitions induced by cisplatin are not clear.In the present letter,we have studied the effect of low-concentration cisplatin on DNA conformation by using AFM imaging.We observed formations of micro-rod structures of linear DNA induced by cisplatin.A method is presented to quantitatively analyze the occurrence of micro-rod structures.We found that the formation of micro-rod structures depends on the DNA sequence.Based on the results,we proposed a physical mechanism to explain the local conformation transitions of DNA molecules under the influence of cisplatin.Cisplatin is the most successful anti-tumor drug, and its pharmacological property is generally considered to derive from the modification of DNA molecules. Structural modifications of short DNA induced by cisplatin have already been investigated. However, the conformation transitions induced by cisplatin are not clear. In the present letter, we have studied the effect of low-concentration cisplatin on DNA conformation by using AFM imaging. We observed formations of micro-rod structures of linear DNA induced by cisplatin. A method is presented to quantitatively analyze the occurrence of micro-rod structures. We found that the formation of micro-rod structures depends on the DNA sequence. Based on the results, we proposed a physical mechanism to explain the local conformation transitions of DNA molecules under the influence of cisplatin.

关 键 词：DNA分子 顺铂 诱导 构象转变 线性 结构改造 原子力显微镜 构象转换 

分 类 号：Q523[生物学—生物化学]