耐甲氧西林金黄色葡萄球菌对碘伏抗性的研究  

STUDY ON THE RESISTANCE OF METHICILLIN- RESISITANT Staphylococcus aureus TO POVIDONE IODINE

作　　者：李双[1] 孙会会[1] 李武平[1] 张晓邦 

机构地区：[1]第四军医大学西京医院 [2]68302部队

出　　处：《中国消毒学杂志》2014年第8期807-809,共3页Chinese Journal of Disinfection

基　　金：陕西省科学技术研究发展计划项目(2110k16-01-07)

摘　　要：目的研究临床分离的耐甲氧西林金黄色葡萄球菌对碘伏消毒剂的抗性变化及其抗消毒剂基因携带情况。方法采用肉汤稀释法和基因检测技术,对临床分离的耐甲氧西林金黄色葡萄球菌抗消毒剂基因携带情况及其对碘伏消毒剂抗性变化进行了观察。结果共检出耐甲氧西林金黄色葡萄球菌152株,其中有11株携带qacA/B基因,携带率为7.24%。碘伏消毒剂对携带qacA/B基因MRSA菌株的MIC值为100-300 mg/L范围,有4株菌的MIC值为300 mg/L。碘伏消毒剂对非携带qacA/B基因的MRSA菌株MIC值为100-200 mg/L范围。结论携带qacA/B基因的MRSA菌株对碘伏消毒剂的抗性有增大趋势,其抗性可能与qacA/B基因有关。Objective To study the resistance of methicillin- resistant Staphylococcus aureus（ MRSA） to Povidone Iodine used in hospital and to investigate the disinfectant- resistant gene qacA /B in meticillin- resisitant Staphylococcus aureus isolated from Xi＇an＇s hospital. Methods The resistance gene was tested by Polymerase Chain Reaction（ PCR） method and tests of minimal inhibitory concentration（ MIC） and minimal bactericidal concentration（ MBC） were used to observe the disinfectant resistance to MRSA between qacA /B gene positive strains and negative strains for Povidone Iodine.Results 152 methicillin- resistant Staphylococcus aureus strains were isolated from four hospitals of Xi＇ an. In those strains,11 strains had the qacA /B gene and the positive rate was 7. 24%; the MIC of Povidone- Iodine for positive gene qacA /B gene strains was between 100 mg /L to 300 mg /L; The MIC of four strains for Povidone- Iodine were 300 mg /L.The MIC of MRSA strains with negative qacA /B gene for Povidone- Iodine was between 100 mg /L to 200 mg /L. Conclusion The resistance of MRSA carrying qacA /B gene for Povidone Iodine has a increasing trend and the resistance is related with qacA /B gene.

关 键 词：耐甲氧西林金黄色葡萄球菌 碘伏消毒剂 抗性 抗性基因 

分 类 号：R187.1[医药卫生—流行病学]