无动物源性成分培养基体外扩增的间充质干细胞  

In vitro expansion of human mesenchymal stem cells in animal serum-free media

作　　者：吴迪[1] 武晓云 吴岩[1] 

机构地区：[1]内蒙古医科大学,内蒙古自治区呼和浩特市010059 [2]北京京蒙高科干细胞技术有限公司,北京市100085

出　　处：《中国组织工程研究》2014年第28期4583-4587,共5页Chinese Journal of Tissue Engineering Research

基　　金：内蒙古自治区科技创新团队资助项目(kjt0020947)~~

摘　　要：背景:间充质干细胞是组织工程、再生医学和免疫抑制治疗领域重要的种子细胞来源,在体外大量扩增成为其进入临床应用的关键环节。目的:对无动物源性成分培养基体外扩增间充质干细胞的研究进展做一综述。方法:由第一作者应用计算机检索2004年1月至2014年4月中国期刊全文数据库(CNKI)及PubMed数据库,英文检索词为"mesenchymal(stromal)stem cells","animal serum-free media","humanized media","human serum","umbilical cord blood serum","platelet rich plasma","platelet lysate","defined medium",中文检索词为"间充质干细胞、无动物血清培养基、人源性培养基、人血清、脐血清、富血小板血浆、血小板裂解液、化学成分明确无血清培养基",最终保留41篇文献。结果与结论:采用人血清、脐血清、富血小板血浆、血小板裂解液以及化学成分明确无血清培养基等可替代胎牛血清,在体外进行间充质干细胞的大规模扩增培养,但是这些培养基添加剂各有其优势和劣势,所以在大规模、系统地应用于临床治疗前,急需解决的问题就是扩增后的功能有效性与移植安全性。BACKGROUND：Mesenchymal stem cells have been an important source of seed cells for tissue engineering, regenerative medicine and immunosuppressive therapy areas. The large expansion is the key to the clinical application of mesenchymal stem cells. OBJECTIVE：To summarize the research progress in the in vitro expansion of human mesenchymal stem cells in animal serum-free media. METHODS：A computer-based online retrieval of CNKI and PubMed databases was performed to search papers published between 2004 and 2014 using the key words of“mesenchymal （stromal） stem cells, animal serum-free media, humanized media, human serum, umbilical cord blood serum, platelet rich plasma, platelet lysate, defined medium”in English and Chinese, respectively. Final y, 41 articles were retained. RESULTS AND CONCLUSION：Human serum, umbilical cord blood serum, platelet rich plasma, platelet lysate and defined medium can replace fetal bovine serum for large-scale expansion of mesenchymal stem cells in vitro. These media or additives have their advantages and disadvantages;therefore, it is urgent to guarantee the functional effectiveness and transplantation safety before large-scale, systematic clinical application of mesenchymal stem cells.

关 键 词：干细胞 间充质干细胞 人血清 脐血清 富血小板血浆 血小板裂解液 化学成分明确无血清培养基 

分 类 号：R318[医药卫生—生物医学工程]