棉花GhRCA1基因启动子的克隆及序列分析  

Cloning and Sequence Analysis of GhRCA1 Gene Promoters from Upland Cotton

作　　者：刘聚波[1] 张玉娟[1] 陈洁[1] 沈法富[1] 

机构地区：[1]作物生物学国家重点实验室山东农业大学农学院,泰安271018

出　　处：《分子植物育种》2014年第4期701-711,共11页Molecular Plant Breeding

基　　金：国家转基因生物新品种培育科技重大专项课题(2008ZX005-004)资助

摘　　要：为了给转基因育种提供诱导型和组织特异型启动子,本研究根据陆地棉GhRCA1基因的cDNA序列和雷蒙德氏棉的基因组序列,采用PCR技术对GhRCA1基因的5'端上游转录调控序列扩增,获得棉花GhRCA1基因的2个启动子序列,分别命名为RCA11和RCA12。生物信息学分析表明,这2个序列均具有多个启动子的基本元件TATA-box和CAAT-box,并含有多种光调控相关的顺式作用元件、激素响应元件、逆境胁迫诱导相关的响应元件等。推测GhRCA1基因的2个启动子都受光的诱导,启动子序列上核苷酸和顺式元件的差异可能使GhRCA1基因的表达受到不同程度的调控。本研究通过克隆和分析GhRCA1基因的启动子序列,为进一步研究该启动子的功能以及基因的表达调控机制奠定了基础。To provide inducible and tissue-specific promoters for transgenic breeding, we try to clone promoters from cotton. According to the cDNA sequence of upland cotton GhRCA1 and the genome sequences of Gossypium raimondii L., the 5＇ end of the upstream transcriptional regulatory sequence of GhRCA1 was amplified by PCR.Two promoter sequences of GhRCA1 were obtained, named as RCA11 and RCA12. Bioinformatics analysis showed that the two promoter regions had many basic transcriptional elements, such as TATA-box and CAATbox, and contained multiple types of light regulating element, hormone response element, and stress-induced response element. We suggest the two promoter regions of GhRCA1 should be light-inducible, and GhRCA1 gene expression may subject to different levels of regulation because of differences in nucleotides and cis-acting elements of promoter sequences. Cloning and analysis of the GhRCA1 promoter region lay the foundation for further functional analysis of the promoter and the regulatory mechanism of the gene expression.

关 键 词：棉花 Rubisco活化酶(RCA) 启动子 克隆 序列分析 

分 类 号：S562[农业科学—作物学] Q943.2[生物学—植物学]