黄瓜黑斑病抗性基因Acu的QTL定位分析  

Mapping and QTL Analysis of Acu Gene Resistance to Cucumber Alternaria Leaf Spot

作　　者：王惠哲[1] 李淑菊[1] 杨瑞环[1] 管炜[1] 邓强[1] 曹明明[1] 

机构地区：[1]天津科润黄瓜研究所,天津300192

出　　处：《分子植物育种》2014年第4期733-739,共7页Molecular Plant Breeding

基　　金：天津市自然科学基金(11JCYBJC09000)资助

摘　　要：黄瓜黑斑病是中国近几年新流行的一种黄瓜叶部病害,目前已成为黄瓜保护地、露地栽培的重要病害之一。国内外关于该病的抗性遗传规律、分子标记等研究很少。本研究以黄瓜感黑斑病自交系P62-1-1和抗黑斑病自交系W43-1-2为亲本构建的F2群体,于苗期进行分生孢子悬浮液喷雾接种鉴定后作为作图群体,利用SSR、SCAR标记技术结合BSA法和遗传图谱法进行基因初步定位,构建了一张包含110个标记、9个连锁群的与黄瓜抗黑斑病相关的遗传图谱。遗传图谱包括101个SSR、6个EST-SSR和3个SCAR标记。该图谱覆盖基因组长度达749.3cM,平均图距为6.81 cM,标记间遗传距离为0.3~21.0cM。每个连锁群上的标记数在3~22个之间,连锁群的长度在20.9~158.5 cM的范围内,平均图距在4.76 cM/marker^10.2 cM/marker之间。结合F2的表型数据,检测到1个控制黄瓜黑斑病抗性的主效QTL位点,位于第5条染色体上,贡献率为81.86%。该项研究为黄瓜黑斑病的分子标记辅助育种、抗病基因的精细定位、图位克隆和进一步的黄瓜功能基因的诠释奠定了基础。Cucumber alternaria leaf spot was one of the new popular foliar diseases in cucumber protected and open field cultivation. Little studies of the genetic rules and markers related to the resistant genes were reported. In this study, F2 population derived from F1 of P62-1-1（susceptible male） and W43-1-2（resistant female） were used as materials, their resistances to alternaria leaf spot were identified through seedling inoculation. To preliminarily locate the resistant genes, SSR, SCAR marker technologies and BSA method, genetic mapping were used in F2 population. We constructed a cucumber genetic map linked to the resistant genes including 110 markers, 9 linkage groups. The markers contained 101 SSR, 6 EST-SSR and 3 SCAR markers. The map spanned 749.3 cM, and map density was 6.81 cM/marker. The smallest genetic distance is 0.3 cM, and the largest is 21.0 cM. Numbers of the markers in each linkage group was from 3 to 22. And the length of the linkage group was between 20.9 and158.5 cM. Considered the phenotypic data of F2, one QTL located in Chromosome 5 was detected, and the contribution rate was 81.86%. The research laid the foundation for the molecular marker-assisted breeding, fine mapping of the disease-resistant genes, map-based cloning and functional genes interpretation.

关 键 词：黄瓜 遗传图谱 黑斑病 QTL 

分 类 号：S436.421.1[农业科学—农业昆虫与害虫防治]