痛风消颗粒对RAW264.7巨噬细胞COX-2和iNOSmRNA调控作用的研究  

On the Regulatory Effects of Gout Granule on RAW264.7 Macrophage COX-2 and iNOS mRNA

作　　者：杨会军[1] 刘维超[2] 李兆福[1] 彭江云[2] 李玲玉[1] 吴晶金[2] 万春平[2] 

机构地区：[1]云南中医学院,云南昆明650500 [2]云南省中医医院,云南昆明650021

出　　处：《风湿病与关节炎》2014年第8期25-28,42,共5页Rheumatism and Arthritis

基　　金：国家自然基金项目(30960476);云南省教育厅科学研究基金项目(2012J016);云南省卫生科技内设研究机构项目(2011WS0026)

摘　　要：目的:研究痛风消颗粒对脂多糖诱导的RAW264.7巨噬细胞炎症是否具有调节作用。方法:采用MTT法筛选干预RAW264.7巨噬细胞的适宜痛风消颗粒含药血清浓度,硝酸还原酶法(Griess法)检测炎症因子一氧化氮(NO)水平变化,RT-PCR法检测诱导型一氧化氮合酶(iNOS)mRNA及环氧化酶2(COX-2)mRNA表达变化。结果:质量分数5%、10%的痛风消颗粒含药血清可降低NO水平,下调COX-2及iNOS mRNA表达。结论:痛风消颗粒能够通过抑制脂多糖诱导的RAW264.7巨噬细胞NO水平,并下调iNOS及COX-2 mRNA表达,对炎症起调控作用。Objective：To study the regulatory effects of Gout Granule on RAW264.7 macrophage inflammation induced by lipopolysaccharide.Methods：The MTT method was used to screen suitable serum concentration containing Gout Granule to interfere RAW264.7 macrophages.The nitrate reductase method （ Griess method ） was used to detect the changes of the level of inflammatory factor nitric oxide （ NO ） .The RT-PCR method was used to detect the changes of the expressions of （ iNOS ） mRNA and （ COX-2 ） mRNA. Results：Serum with the mass fraction of 5% and l 0% containing Gout Granule could decrease the level of NO and down regulate the expressions of COX-2 and iNOS mRNA.Conclusion：Gout Granule could regulate inflammation by inhibiting the level of NO of LPS-induced RAW264.7 macrophages and down-regulating the expressions of iNOS and COX-2 mRNA

关 键 词：痛风消颗粒 大鼠 含药血清 巨噬细胞 环氧化酶2 诱导型一氧化氮合酶 

分 类 号：R285[医药卫生—中药学]