鸡新城疫、传染性支气管炎二联活疫苗耐热保护剂筛选和优化  

Selection and optimization of thermostable agent for Newcastle Disease and Infectious Bronchitis bivalent vaccine

作　　者：陈生雷 李凤艳 高洪霞 李沂 王岩[2] 舒秀伟 

机构地区：[1]辽宁益康生物股份有限公司,辽宁辽阳111000 [2]辽宁省锦州市黑山县动物疫病预防控制中心,辽宁黑山121400

出　　处：《现代畜牧兽医》2014年第8期1-4,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：应用冻干制品加速热稳定试验，测试7组冻干保护剂配方对鸡新城疫、传染性支气管炎二联活疫苗（LaSota株＋H120株）的耐热保护效果。试验筛选出用于鸡新城疫、传染性支气管炎二联活疫苗的耐热保护剂，使用该保护剂的疫苗表现为37℃保存10d，病毒含量下降0．4个滴度，常规疫苗下降2～2．4个滴度；25℃保存30d，病毒含量下降0．4～0．6个滴度，常规疫苗下降1．8～2．6个滴度。证明此保护剂具有良好的耐热效果。In this paper, we tested protective effects of 7 groups of the lyoprotectant recipe for Newcastle Disease and Infectious Bronchitis Vaccine, Live Vaccine （La Sota strain ＋ H120 strain） were used for cross reference by an accelerated stability test. The best lyoprotectant recipe of Newcastle-Bronchitis Vaccine had been screened in this experiment, and the results indicated that when exposure this lyoproteetant vaccine at 37 ℃ for 10 days, or exposure at 25℃ for 30 days, the vaccine virus titers declined by 0.4 unit or 0.4-0.6 unit respectively, while other lyoprotectant vaccines virus titers decreased by 2-2.4 unit or 1.8-2.6 unit respectively. In conclusion, the new lyoprotectant showed effective protection for Newcastle-Bronchitis Vaccine.

关 键 词：活疫苗 耐热保护剂 热稳定性试验 

分 类 号：S858.31[农业科学—临床兽医学]