LC-MS/MS法同时测定人血浆中4种茶碱类药物浓度  

Simultaneous determination of four theophylline drugs in human plasma by LC-MS/MS

作　　者：肖昌钱[1] 李贺[1] 李卫[1] 李力[1] 

机构地区：[1]浙江医院药剂科浙江省老年医学重点实验室,杭州310013

出　　处：《中国临床药理学杂志》2014年第8期724-726,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家科技重大专项基金资助项目(2013ZX09303005);国家自然科学基金资助项目(31201040)

摘　　要：目的：建立同时测定人血浆中茶碱、多索茶碱、二羟丙茶碱和氨茶碱浓度的LC-MS/MS法。方法人血浆样品用乙腈直接沉淀蛋白后，用ZORBAX SB C18色谱柱，以乙腈-10 mmol· L-1乙酸铵溶液（均含0.1％甲酸）为流动相梯度洗脱，用电喷雾离子化源，正离子方式，多反应监测（ MRM）扫描方式进行监测。结果血浆中茶碱、多索茶碱、二羟丙茶碱和氨茶碱的线性范围为0.10～50.00μg· mL-1（ r ＞0.99），定量下限为0.10μg · mL-1；平均回收率93.5％～109.8％；日内、日间精密度（ RSD）均小于15％；提取回收率在81.2％～90.8％。结论该方法快速、灵敏、准确，专属性强，重复性好，适用于人血浆中4种茶碱类的药代动力学研究。Objective To develop an LC -MS/MS method for simulta-neous determinations of theophylline , doxofylline diprophylline and amin-ophylline in human plasma.Methods The plasma samples were precipi-tated by acetonitrile.The separation was achieved on ZORBAX SB C 18 col-umn and eluted with linear gradient using acetonitrile -10 mmol · L-1 mammonium acetate , both of which were acidified with 0.1%formicacid.Detection of the analytes were made using positive ion electrospray ioniza -tion （ ESI） in the multiple reaction monitoring （ MRM） mode.Results The linear calibration curve of theophylline , doxofylline , diprophylline and aminophyllinewere obtained concentration range of 0.10 -50.00 μg · mL-1 （r＆gt;0.99）, with the lower limit of quantitation at 0.10 μg· mL-1.The average recovery was between 93.5% and 109.8%, Intra -day and inter-day relative standard deviations were both below 15%.The recoveries were 81.2%-90.8%.Conclusion This method is rapid, sensitive, accu-rate, specific and reliable, suitable for simultaneous determination of theo-phylline, doxofylline, diprophylline and aminophylline in human plasma.

关 键 词：茶碱 多索茶碱 二羟丙茶碱 氨茶碱 液质联用法 血药浓度 

分 类 号：R917[医药卫生—药物分析学] R974.3[医药卫生—药学]