缫丝蚕蛹蛋白酶法水解条件优化  

Optimization of protease hydrolysis conditions on silkworm pupa protein

作　　者：钟雨珅 罗金凤[1] 黄先智[2] 丁晓雯[1] 

机构地区：[1]西南大学食品科学学院,重庆市农产品加工及贮藏重点实验室,重庆400715 [2]西南大学蚕学与系统生物学研究所,重庆400715

出　　处：《食品工业科技》2014年第17期232-235,240,共5页Science and Technology of Food Industry

基　　金：国家桑蚕产业技术体系蚕茧综合利用项目(CARS-22-ZJ0503);公益性行业(农业)科研专项项目(201303053)

摘　　要：目的:利用酶水解缫丝蚕蛹蛋白,拟为其深度开发利用提供参考。方法:利用蛋白酶水解缫丝蚕蛹蛋白,并用分光光度法测定各指标。结果表明:单酶水解时,实验所用的8种酶中以蚕蛹蛋白水解专用酶的水解度最高,最佳水解条件是底物浓度7%,温度53℃,pH7.2,加酶量3%,水解度达到21.44%。Purpose. Enzyme hydrolysis of silkworm pupa protein was studied in order to provide references to deeply exploit silkworm pupa protein.Methods. Protease was used to hydrolyze silkworm pupa protein and spectrophotometry was used to detect indicators.Results：When hydrolyzed by single protease,dedicated protease for silkworm pupa protein showed the highest hydrolysis degree out of 8 proteases used in the experiment, the best hydrolysis conditions were：the substrate concentration was 7% ,53℃, pH7.2,the additive amount of protease was 3% ,and the hydrolysis degree turned out to be 21.44%.

关 键 词：缫丝蚕蛹 酶水解 条件优化 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]