利巴韦林对人肺腺癌细胞A549、人乳腺癌细胞Hela迁移和凋亡的影响研究  

Effects of Ribavirin on the Migration and Apoptosis of Human Lung Adenocarcinoma A549 Cells and Human Breast Cancer Hela Cells

作　　者：郑来双 李帅[2] 马玉莲 

机构地区：[1]河南省医药采购服务中心,郑州450003 [2]河南省肿瘤医院,郑州450008

出　　处：《中国药房》2014年第33期3103-3106,共4页China Pharmacy

摘　　要：目的:研究利巴韦林对人肺腺癌细胞A549、人乳腺癌细胞Hela迁移和凋亡的影响。方法:以A549、Hela细胞为模型细胞,分别分为对照组和利巴韦林低、中、高剂量(20、40、80μmol/ml)组,作用24 h后检测各组细胞的存活率、迁移能力、凋亡情况、酸性膜泡积累情况、自噬标志蛋白LC3(以LC3-Ⅱ/LC3-Ⅰ为指标)的表达情况。结果:与对照组比较,利巴韦林能降低A549、Hela细胞的存活率(中、高剂量组差异均有统计学意义,P<0.05或P<0.01)和迁移能力,增加A549、Hela细胞的凋亡率(低、中、高剂量差异均有统计学意义,P<0.05或P<0.01)、酸性膜泡积累数量与剂量呈反相关,LC3-Ⅱ/LC3-Ⅰ比值(中、高剂量差异均有统计学意义,P<0.01),作用与剂量呈正相关。结论:利巴韦林可能通过诱导细胞自噬降低A549、Hela细胞的迁移并诱导其凋亡。OBJECTIVE： To study the effects of ribavirin on the migration and apoptosis of human lung adenocarcinoma A549 cells and breast cancer Hela cells. METHODS： A549 cells and Hela cells were taken as model cells and divided into control group, ribavirin low-dose, medium-dose and high-dose groups （20, 40, 80 μmol/ml）. The survival rate, migration ability, cell apoptosis, the accumulation of acid membranous vesicles, the expression of cell autophagy marker LC3 （LC3-Ⅱ/LC3- Ⅰ ） were determined 24 h later. RESULTS： Compared with control group, ribavirin could decrease the survival rate （there was statistical significance of me- dium-dose and high-dose groups, P〈0.05 or P〈0.01） and migration ability of 549 cells and Hela cells; also could increase the apoptosis rate of them （there was statistical significance of low-dose, medium-dose and high-dose groups, P〈0.05 or P〈0.01）, the number of acid membranous vesicles and LC3- Ⅱ/LC3- Ⅰ （there was statistical significance of medium-dose and high-dose, P〈 0.01）; the effects were positively associated with dosage. CONCLUSIONS： Ribavirin can reduce the migration and induce apoptosis of A549 and Hela cells by inducing cell autophagy.

关 键 词：利巴韦林 人肺腺癌细胞A549 人乳腺癌细胞Hela 迁移 凋亡 

分 类 号：R965[医药卫生—药理学]