黄原胶对脂多糖诱导兔软骨细胞氧化损伤的影响  

Effect of Xanthan Gum on Oxidative Damage in Rabbit Chondrocyte Induced by Lipopolysaccharides

作　　者：赵丽娟[1,2] 凌沛学[1,2] 

机构地区：[1]山东大学药学院,山东济南250012 [2]山东省生物药物研究院博士后科研工作站,山东济南250101

出　　处：《药物生物技术》2014年第2期121-125,共5页Pharmaceutical Biotechnology

基　　金：国家重大新药创制专项"国家山东创新药物孵化基地"支持项目(No.2010ZX09401-302)

摘　　要：考察不同浓度(50,100,200μg/mL)黄原胶(XG)对脂多糖(LPS)刺激的兔膝骨关节软骨细胞氧化损伤的影响。玻璃酸钠(SH)、维生素C(Vc)作为阳性对照药,采用流式细胞术和荧光酶标仪检测活性氧(ROS)表达,试剂盒检测一氧化氮(NO)、丙二醛(MDA)、超氧化物歧化酶(SOD)的含量。两种ROS检测方法显示,与模型组相比,XG各剂量组均能降低ROS水平,XG与SH无显著性差异,Vc的抗氧化能力强于XG及SH。与模型组相比,XG、SH均能减少LPS诱导软骨细胞产生的NO,SH较为显著。XG、SH能显著降低LPS诱导产生的MDA,高剂量组XG优于SH。XG能增强LPS诱导兔软骨细胞产生SOD的活性。综上所述,XG能逆转LPS造成的兔软骨细胞氧化损伤,消除ROS,提高SOD活性,降低MDA、NO水平,是OA的潜在治疗药物。Xanthan gum（XG） is a kind of microbial polysaccharide with great economical value and is extensively used in food, petroleum, cosmetics ,and so on. The purpose of this article was to investigate the effect of XG（ 50,100,200 μg/mL） on oxidative damage in rabbit chondrocyte induced by lipopolysaccharide（ LPS, 10 μg/mL）. And the drug in positive control group was replaced by sodium hyaluronate （SH） and vitamin C （Vc）. The effect of XG on ROS was measured by fluorence microplate reader and flow cytomety. And the effect of XG on the expression of NO, MDA, SOD was determined by test kits. The results showed that compared with control group, model group could increase the expression of ROS, NO, MDA and decrease the activity of SOD, so that the cell model of OA in vitro was successful and practicable. The results of two different testing ROS methods showed that LPS with different concentrations of XG induced the decreased ROS expression when compared with model group, but no significant differences were observed between SH and XG. And compared with XG and SH,Vc had a stronger ability to reduce ROS expression. Compared with model group,XG and SH reduced significantly the expression of NO, and SH had a stronger ability than XG. XG and SH could decrease the level of MDA,and the higher dose group of XG（200 μg/mL） was much better than SH. XG could increase the expression of SOD in LPS-induced rabbit chondrocyte, so that XG had an ability to downturn the oxidative damage in rabbit chondrocyte induced by LPS. XG could eliminate ROS, improve the activity of SOD, and lower the level of MDA and NO. Then XG could be a new drug to treat OA in the future.

关 键 词：骨关节炎 黄原胶 软骨细胞 脂多糖 活性氧 一氧化氮 丙二醛 超氧化物歧化酶 

分 类 号：R965[医药卫生—药理学]