亚慢性镉中毒对育成鸡卵巢酶活性的影响  

Studies on Sub-chronic Cadmium Poisoning to the Enzyme Histochemistry of Chicken's Ovary

作　　者：杨淑华[1,2] 何剑斌[1] 于立辉[1] 李林[1] 杨娜[1] 徐世文[2] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110161 [2]东北农业大学动物医学院,哈尔滨150030

出　　处：《沈阳农业大学学报》2014年第2期232-235,共4页Journal of Shenyang Agricultural University

基　　金：黑龙江省教育厅项目(10551038)

摘　　要：为建立育成鸡镉中毒模型,以雌性育成鸡为研究对象,饲喂不同浓度的CdCl2(140mg·kg-1和210mg·kg-1)之后进行临床观察,病理学和卵巢酶组织化学的检测,深入探讨镉对鸡卵巢组织损伤的机理。结果表明:卵巢组织SDH、ALP、Ca2+-ATPase、G-6-Pase、Mg2+-ATPase等酶活性随染镉剂量增大、时间延长呈降低趋势,高镉组酶活性降低(+或+/-)较低镉组组织酶活性(++或+)显著;卵巢组织ACP活性随染镉剂量增大、时间延长呈现升高趋势。镉可改变卵巢组织细胞生物膜上的多种酶的活性,损伤细胞生物膜,破坏酶赖以存在的系统,影响卵巢的发育。In order to study the effects of cadmium on the enzyme histochemistry, female adult chicken fed with adding 140mg· kg^-1CdCl2 and 210mg·kg^-1CdCl2 individually to the complete feed were used as cadmium poisoning models. The results showed that cadmium influenced enzyme activity of the femal Chick＇s ovary, decreased the activity of SDH, ALP, G-6-Pase, Ca^2＋- ATPase, MgZ^2＋-ATPase, increased the activity of ACP, interfered tissue metabolism, and depressed the developing of ovarian follicle. Cadmium poisoning played significantly inhibitory roles in enzyme of ovary tissues, affected the activity of many kinds of enzymes in cells and injured the skeleton of biofilm.

关 键 词：镉 育成鸡 卵巢 酶活性 

分 类 号：TK6[动力工程及工程热物理—生物能]