香青兰总黄酮在大鼠体内的药代动力学研究  

Study on the Pharmacokinetic Behavior of Dracocephalum Moldavica L.Flavonoids in Rat

作　　者：袁勇[1] 邢建国 王立萍[3] 姚佳茗 王新春[1,3] 

机构地区：[1]石河子大学医学院第一附属医院,石河子832002 [2]新疆维吾尔自治区药物研究所,乌鲁木齐830004 [3]石河子大学药学院,石河子832002

出　　处：《石河子大学学报（自然科学版）》2014年第1期69-72,共4页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金新疆联合基金重点项目(U1203204);国家"重大新药创制"科技重大专项(2012zx09102201-009)

摘　　要：为探讨香青兰总黄酮在大鼠体内的药代动力学。采用以田蓟苷为指标,HPLC法测定大鼠灌胃给药后血浆中田蓟苷的浓度,并采用DAS2.0软件计算药代动力学参数。结果显示,血浆中田蓟苷浓度在0.031～39.68μg/mL范围内线性关系良好（R=0.9990）,日内精密度（RSD）〈5%,日间精密度（RSD）〈10%,方法回收率在98.01%～103.23%之间,提取回收率在72.67%～90.35%之间。香青兰总黄酮在大鼠体内呈二室分布,大鼠灌胃香青兰总黄酮提取物3个剂量（300,600,1200 mg/kg）后,t1/2β分别为（6.904±0.922）,（6.512±3.302）,（9.820±3.116）h,AUC（0-t）分别为（0.527±0.018）,（0.980±0.097）,（1.215±0.108）mg/L/h,Tmax分别为（0.292±0.083）,（0.139±0.048）,（0.222±0.048）h,Cmax分别为（0.177±0.018）,（（0.451±0.064）,（0.656±0.115）mg/L。由此可知,该法适用于测定香青兰总黄酮在大鼠体内的血药浓度并进行药代动力学研究。To study pharmacokinetics of Dracocephalum Moldavica L.Flavonoids in rat. Plasma concentration of tilianin was used as the indicators,which was measured by HPLC.The pharmacokinetic parameters were calculated by DAS2.0.It was showed good linear relationship when the concentration of tilianin in the range of 0.031 ～39.68 μg/mL with the correlation coefficient of 0.9990.The intra-day precision of this method was less than 5%,and the inter-day precision was less than 10%.The accuracy was 98.01%～103.23%.The extraction recovery was 72.67%～90.35%.The main pharmacokinetics parameters of tilianin after oral administration with three different doses（300,600,1200 mg/kg） to rats were： t1/2β：（6.904±0.922）,（6.512±3.302）,（9.820±3.116）h; AUC（0-t）：（0.527±0.018）,（0.980±0.097）,（1.215±0.108） mg/L/h;Tmax：（0.292±0.083）,（0.139±0.048）,（0.222±0.048）h;Cmax：（0.177±0.018）,（0.451 ±0.064）,（0.656 ±0.115） mg/L.The method is proved to be suitable for the pharmacokinetic study of Dracocephalum Moldavica L.Flavonoids in rats.

关 键 词：香青兰总黄酮 药代动力学 高效液相色谱法 

分 类 号：R283[医药卫生—中药学]