外源NO对铜胁迫下番茄植物螯合肽及精氨酸代谢的影响  

Effect of Exogenous NO on Phytochelatins and Arginine Metabolism in Tomato Under Copper Stress

作　　者：王建[1] 于世欣[1] 王逸筠 窦巧惠 崔秀敏[1] 

机构地区：[1]山东农业大学资源与环境学院,山东泰安271018

出　　处：《水土保持学报》2014年第4期317-323,共7页Journal of Soil and Water Conservation

基　　金：国家自然科学基金项目(31201619)

摘　　要：一氧化氮(NO)作为生物活性分子,广泛参与各种生物、非生物胁迫。采用营养液培养,研究铜胁迫下外源NO对番茄体内植物螯合肽及精氨酸代谢的影响。结果表明,与CK相比,铜胁迫可以显著激活番茄体内γ-ECS、GS活性,根系GSH、PCs含量急剧升高,且随着处理时间的延长,γ-ECS、GS酶活性、GSH、PCs含量呈持续上升趋势;同时促进植物体内精氨酸代谢增加多胺合成。添加外源SNP,进一步提高铜胁迫下番茄根系γ-ECS、GS活性,促进GSH、PCs的合成,促进番茄多胺的合成。铜胁迫下,外源NO可能启动了某些信号机制,并通过激活或增强某些酶促和非酶促系统,降低过多Cu2+的生物毒性和氧化伤害。Nitric oxide （NO）, as a biologically active molecule, widely involved in the biotic and abiotic stresses. By using solution culture, the dynamic changes in phytochelatins and arginine metabolism mediated by exogenous NO in tomato were studied. The results showed that compared with CK, the activity of 7--ECS and GS significantly activated, and sharply rose in GSH and PCs content in tomato root. Moreover 7--ECS and GS activity, GSH and PCs content constantly rose as the extension of processing time under copper stress. Adding exogenous SNP could further enhance the improve γ--ECS and GS activity in tomato, and promote the production of PAs by promoting arginine metabolism. Under copper stress, exogenous NO might start a certain signal mechanism, and through activate or enhance of enzyme or non-enzymatic system in the GSH-PCs synthetic way, to reduce biological toxicity and oxidative damage induced by excess Cu2＋.

关 键 词：SNP 精氨酸 植物螯合肽 铜胁迫 番茄 

分 类 号：S154.4[农业科学—土壤学] S641.2[农业科学—农业基础科学]