苦豆子愈伤组织的诱导及去褐化处理  

Callus Induction and Antibrowning in Subculture of Sophora alopecuroides L.

作　　者：陈亚萍[1] 高媛[1] 顾沛雯[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《北方园艺》2014年第16期96-99,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(31260452)

摘　　要：以苦豆子为试材,采用组织培养的方法,研究了外植体种类、激素水平、水解酪蛋白浓度等不同因子对愈伤组织生长状态的影响,同时对愈伤组织进行了去褐化处理。结果表明:苦豆子子叶为诱导愈伤组织的最佳外植体;最佳诱导培养基为MS+2,4-D 0.5mg/L+NAA0.5mg/L+6-BA 1.0mg/L;最佳继代培养基为MS+2,4-D 1.0mg/L+6-BA 4.0mg/L+NAA1.0mg/L+CH 1 000mg/L;添加3%蔗糖的条件下快速继代是较好的去褐化措施。Taking Sophora alopecuroides as material,the effect of explants type,hormone levels,casein hydrolysate on the growth of callus were measured by tissue culture method. Meanwhile many measures were carried out to prevent browning. The results showed that the cotyledon was the optimum explants of callus induction,the best medium of callus induction was MS＋2,4-D 0. 5 mg/L＋NAA 0. 5 mg/L＋6-BA 1.0 mg/L;and the best subculture medium was MS＋ 2,4-D 1.0 mg/L＋6-BA 4. 0 mg/L＋NAA 1.0 mg/L＋CH 1 000 mg/L;the best proposal was frequent subculture with 3% sucrose.

关 键 词：苦豆子 愈伤组织 继代培养 去褐化处理 

分 类 号：Q946.8[生物学—植物学]