二化螟高毒力苏云金杆菌菌株的筛选  

The Screening of High Virulence Bacillus thuringiensis Strains to Chilo suppressalis(Walker)

作　　者：张鑫琳[1] 于洪春[1] 房丹丹[1] 项丹丹[1] 许振南 许国庆[2] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]辽宁省农业科学院植物保护研究所,辽宁沈阳110161

出　　处：《北方园艺》2014年第16期112-114,共3页Northern Horticulture

基　　金：国家公益性行业(农业)科研专项资助项目(201003025)

摘　　要：以高毒力苏云金芽孢杆菌（Bacillus thuringiensis,Bt）为供试菌株,以二化螟为试虫,初步研究了30个Bt菌株对1-2龄二化螟幼虫的毒力效果,进而以筛选出的5株较高青力Bt菌株进行2-3龄二化螟幼虫青力试验,以筛选出适合黑龙江省二化螟防治的Bt菌株。结果表明：Bt14菌株在饲喂菌液168h后,二化螟的校正死亡率可达90%以上,毒力回归方程为y=0.6121x＋0.8789,R2=0.9989,LC50=5.4×106芽孢/mL,致死剂量与其它菌株差异显著,表明该菌株对防治二化螟具有很好的应用前景。Taking Chilo suppressalis as material,using high virulence Bt strains, the effect of 30 Bt strains on the virulence test of 1-2 instar Chilo suppressalis larvaes were studied,then the effect of 5 high virulence Bt strains that screened out on virulence of 2 - 3 instar Chilo supprealis larvaes were studied, to screen out suitable high virulence Bt strain to prevention Chilo suppressalis in Heilongjiang province. The results showed that the corrected mortality of Chilo suppressalis was 90% treated by Bt14 bacteria liquid after 168 h. And then its toxicity regression equations were：y= 0. 6121x＋0. 8789,R2 =0. 9989. Its LG50 was 5.4 × 10^6 spores/mI.,which was significant difference from other Bt stains. The results suggested that Bt14 could have a good prospect for controlling Chilo suppressalis.

关 键 词：二化螟 苏云金杆菌 菌株 生物测定 毒力 筛选 

分 类 号：S435.112.1[农业科学—农业昆虫与害虫防治]