牛耳枫枯梢病病原分离与鉴定  

Isolation and Identification of the Shoot Blight Pathogen on Daphniphyllum calycinum

作　　者：胡凤云[1] 刘威[1] 叶云峰[1] 蒋妮[1] 刘丽辉[1] 黄雪彦[1] 

机构地区：[1]广西壮族自治区药用植物园广西药用资源保护与遗传改良重点实验室,广西南宁530023

出　　处：《北方园艺》2014年第16期115-118,共4页Northern Horticulture

基　　金：广西科学研究与技术开发计划资助项目(桂科攻1099063-15)

摘　　要：以染病及健康的牛耳枫为试材,采用常规组织分离法对牛耳枫枯梢病病原进行分离,通过对病原菌的形态学特征、致病性测定和rDNA-ITS序列分析,对引起牛耳枫枯梢病的病原菌进行了鉴定。结果表明:牛耳枫枯梢病的病原为尖孢镰刀菌(Fusarium oxysporum),该病原菌直接侵染牛耳枫嫩梢引起枯梢病。Taking healthy and infected Daphniphyllum calycinum as maLerial,the pathogen caused shoot blight of Daph niphyllum calycinum was isolated by general tissue isolation, and identified based on morphological characteristics, patho genicity test and rDNA-ITS sequences analysis. The results showed that the pathogen caused shoot blight of Daph niphyllum calycinum was Fusarium oxysporum ,and F. oxysporum causing shoot blight by directly infecting on Daphniphyllum calycinum.

关 键 词：牛耳枫 枯梢病 尖孢镰刀菌 病原鉴定 

分 类 号：S435.67[农业科学—农业昆虫与害虫防治]