不同碳源对白及种子萌发和幼苗生长的影响  

The Effect of Different Carbon and Dosage on Seed Germination Characteristics and Growth of Seedling for Bletilla striata

作　　者：李青风[1,2,3] 桂阳[2,3] 陈娅娅[2,3] 张丽娜[2,3] 朱国胜[1,2] 白志川[1] 

机构地区：[1]西南大学园艺园林学院,重庆北碚400700 [2]贵州省现代中药材研究所,贵州贵阳550006 [3]贵州省农业生物技术重点实验室,贵州贵阳550006

出　　处：《北方园艺》2014年第16期150-154,共5页Northern Horticulture

基　　金：国家科技支撑计划资助项目(2009BAI74B02);贵州省优秀青年科技人才培养对象专项资金资助项目[黔科合人字(2011)35号];贵州省科技计划资助项目[黔科合院所创能(2010)4002];中央补助地方科技基础条件专项基金资助项目[黔科条中补地(2010)4002];贵州省农业科学院博士科研启动资助项目(黔农科院人才启动项目[2009]006号);贵州省农业科学院专项资助项目(黔农科院院专项[2010]037号)

摘　　要：以白及种子为试材,利用3种碳源(葡萄糖、蔗糖、可溶性淀粉)和2种用量(30、60g/L)采用多因素随机区组试验设计进行组织培养,分析不同碳源对白及种子萌发和幼苗生长的影响。结果表明:可溶性淀粉和葡萄糖可以促进种子膨大变绿,用量不存在显著差异;而蔗糖能显著提高幼苗的株高、叶长、叶宽、鲜重和根长;最初播种可选择可溶性淀粉作为碳源(用量为30g/L),适合幼苗生长的最佳碳源为蔗糖;该研究筛选出适合白及种子萌发和幼苗生长的最佳碳源及用量,为缩短育苗时间奠定了基础。Taking the seeds of Bletilla striata as materials,three carbon sources （glucose,sucrose and soluble starch） and two levels （30 g/L and 60 g/L） were choosen for randomized block design. Statistics analysis of seed germination and seedling growth were used to select appropriate carbon sources. The results showed that glucose and soluble starch promoted the embryo enlargement and greening,and no significance between two factors. Sucrose significantly increased the plant height,length and width of leaves,fresh weight,and root length. Soluble starch （30 g/L） was used for seed germination as best source,and sucrose as best source for seedling growth. The findings suggested that appropriate carbon source was very useful for seed germination and seedling growth of Bletilla striata.

关 键 词：白及 碳源 种子萌发特性 幼苗生长 

分 类 号：S682.31[农业科学—观赏园艺]