人永生化尿路上皮细胞系与胶原支架共培养的实验研究  

Study of combination of immortalized human bladder urothelium cell line with collagen scaffold

作　　者：周哲[1] 赵阳[1] 吴稼晟[2] 张明[1] 李伟[2] 孙康[2] 王忠[1] 卢慕峻[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院泌尿外科,上海200011 [2]上海交通大学材料科学与工程学院金属基复合材料国家重点实验室,上海200240

出　　处：《中国现代医学杂志》2014年第20期6-9,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:81370860);国家自然科学基金(No:81070605);上海交通大学"医工(理)交叉研究基金"(No:YG2011MS14);上海市教委科研创新项目(No:09YZ76)

摘　　要：目的观察评价人永生化尿路上皮细胞系(immortalized human bladder urothelium cell line)SVHUC-1在胶原支架中的生长情况,为进一步体内组织修复研究提供依据。方法制备疏松多孔的胶原支架材料,SV-HUC-1传代扩增后,接种在胶原支架上,体外培养7 d后取材,观察SV-HUC-1和胶原的复合情况。结果 SV-HUC-1呈"多角形"、"铺路石"样,以克隆团形式生长。HE染色显示胶原支架上的SV-HUC-1经过体外培养7 d后即可形成沿支架表面排列的复层上皮结构,大量SV-HUC-1渗透到胶原的空隙内。扫描电镜显示细胞材料复合良好。结论 SV-HUC-1能在胶原支架上较好的生长,胶原可以作为理想的支架材料,进一步用于组织工程膀胱修复的研究。[ Objective] To observe the growth of immortalized human bladder urothelium cell line （SV- HUC-1） on collagen biomaterials cultured in vitro. [Methods] Porous collagen scaffolds was prepared. After in vitro culture and amplification, the SV-HUC-1 were seeded onto the collagen scaffolds. After cultured for 7 days, the cellscaffolds composites were observed. [Results] SV-HUC-1 were polygon and paving stonelike, and they could form the clone groups. After 1 week of in vitro culture, the SV-HUC-1 formed a muftilayer structure on the surface of the scaffolds, and a great deal of cells grew into the scaffolds. Scanning electron microscopy revealed that cell and scaffolds composited well. [Conclusion] Collagen scaffolds have a good biocompatibility with SV-HUC-1, which can be used as perfect scaffolds in tissue defect reconstruction with the application of tissue engineering.

关 键 词：尿路上皮细胞 胶原 支架 组织工程 

分 类 号：Q813.11[生物学—生物工程]