乳岩宁方剂逆转多药耐药MCF-7/Adr细胞的耐药机制的研究  

Resistance mechanism of Ruyanning prescription on reversing multi-drug resistant MCF-7/Adr cell

作　　者：陈卓[1] 于卫江[1] 

机构地区：[1]郑州大学附属肿瘤医院(河南省肿瘤医院)药剂科,河南郑州450008

出　　处：《中国现代医学杂志》2014年第21期11-14,共4页China Journal of Modern Medicine

摘　　要：目的观察非细胞毒性浓度中药乳岩宁方水煎剂对耐阿霉素的乳腺癌细胞MCF-7/Adr多药耐药的逆转作用,并探讨其逆转机制。方法将细胞悬液接种于96孔培养板中,细胞数为2×108/mL,乳岩宁水煎剂(2.5、5、10、20及40μg/mL)处理MCF-7/Adr细胞72 h后,采用四甲基偶氮唑盐(MTT)比色法测定乳岩宁方水煎剂的细胞毒性及其对MCF-7/Adr细胞敏感性的影响,用蛋白印迹法检测非细胞毒性浓度的乳岩宁方水煎剂处理后MCF-7/Adr细胞中ERCC1的表达。结果乳岩宁方水煎剂对MCF-7/Adr细胞有一定的细胞毒作用,其非细胞毒性质量浓度为6.25μg/mL,非细胞毒性浓度乳岩宁方水煎剂可显著降低阿霉素对MCF-7/Adr细胞的半数抑制浓度(IC50),其IC50由原来的(8.75±0.39)μg/mL降低至(4.14±0.21)μg/mL,其逆转倍数为2.11倍;乳岩宁方水煎剂作用于MCF-7/Abr细胞后,其ERCC1的水平显著下降(P<0.01)。结论乳岩宁方水煎剂可部分逆转多药耐药细胞MCF-7/Abr对阿霉素的耐药性,其作用机制可能是通过降低细胞内ERCC1的表达水平从而降低其耐药性。[ Objective ] To observe the reverse effect of non-cytotoxic concentration Ruyanning water decoction on multi-drug resistance of the adriamycin-resistant breast cancer cell MCF-7/Adr and explore its reverse mechanism. [Methods] Cell suspension was inoculated into a 96-well culture plate with the number of ceils 2xl0S/mL. Then MCF-7/Adr cell was treated with Ruyanning water decoction for 72 h, at the same time, the cytotoxicity of Ruyanning water decoction was examined by MTr（methyl thiazolyl tetrazolium） assay method and its effect on MCF- 7/Adr cell chemotherapy was also analyzed. After disposal of the non-cytotoxic concentration Ruyanning water decoction, ERCC 1 expression in MCF-7/Adr cell was examined by Western blotting method. [ Results ] Ruyanning water decoction had cytotoxic effect on MCF-7/Adr cell with a non-cytotoxic mass concentration of 6.251xg/mL. On the other hand, Ruyanning water decoction with non-cytotoxicity concentration could significantly lower adriamycin on median inhibitory concentration of MCF-7/Adr cell, whose IC50 decreased from （8.75±0.39）μg/mL to （4.14±0.21） μg/mL. The reversal index was 2.11. When Ruyannig water decoction had acted on MCF-7/Adr cell, its ERCC l level also significantly decreased （P 〈0.01）. [Conclusions] Ruyanning water decoction could partly reverse drug resistance of the multi-drug resistant cell MCF-7/Adr on adriamycin, and its mechanism may be through the reduction of ERCC1 expression level in cells, the reduction of drug resistance was achieved.

关 键 词：乳岩宁方水煎剂 MCF-7 Abr细胞 多药耐药 切除修复交叉互补基因1(ERCC1) 

分 类 号：R917[医药卫生—药物分析学]