关苍术毛状根培养体系建立及其多糖含量测定  

Establishment of Culture System of Atractylodes japonica Koidz. ez Kitam. Hairy Roots and Determination of Polysaccharide

作　　者：吕政[1] 张淑丽[1] 路放[1] 杨世海[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《中国药学杂志》2014年第16期1386-1392,共7页Chinese Pharmaceutical Journal

基　　金：科技部科技支撑计划项目(2011BAI03B01)

摘　　要：目的建立关苍术毛状根的诱导方法和培养体系,并对关苍术中多糖含量进行分析。方法用发根农杆菌R1000和R1601菌株感染关苍术叶、茎、根外植体,诱导毛状根产生,对扩大培养体系进行优化,并对毛状根进行PCR检测,利用紫外分光光度计测定关苍术中多糖的含量。结果 R1000感染叶片诱导率最高为(62±2.58)%,最佳增值培养基为White,应用PCR证实R1000和R1601 Ri质粒上的rolB基因片段以成功转入被感染的关苍术叶和根中。结论关苍术毛状根多糖含量为(126.7±16.2)mg·g-1。OBJECTIVE To establish the hairy roots culture system of Atractylodes japonica Koidz. ez Kitam. and study the hairy roots growth and analyze the polysaecharide content in hairy roots culturing system. METHODS The leaves, stalks, and roots were infected with two Agrobacterium rhizogenes strains R1601 and R1000 to induce hairy roots, the expansion of the training system was op- timized, and the hairy roots were detected by PCR. The purity of polysaccharide in Atractylodes japonica Koidz. ez Kitam was deter- mined by UV. RESULTS The induction rate of strain R1000 for leaves could reach as high as （62 ± 2. 58 ） %. The value-added cul- ture medium was White. The PCR examination result showed that rol B genes were successfully inserted into the hairy roots of Atrac- tylodes japonica Koidz. ez Kitam. CONCLUSION The content of polysaeeharide was （ 126. 7 ± 16. 2） mg·g^-t in the hairy roots.

关 键 词：关苍术 毛状根 发根农杆菌 多糖含量 

分 类 号：R284[医药卫生—中药学]