稻蝗属部分种类核DNA ITS1区序列系统发育关系  

Molecular Phylogeny of Oxya Species Inferred from rDNA ITS1 Sequences

作　　者：任鸿雁[1] 任竹梅[1] 马恩波[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2014年第3期429-433,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(31170359);山西省自然科学基金(2012011034-4);山西省回国留学人员科研资助项目(2013-020)

摘　　要：以核DNA ITS1(Internal Transcribed Spacer 1)序列作为分子标记,对我国稻蝗属部分物种种间相互关系进行初步探讨。对获得的7种稻蝗ITS1区序列进行同源性比较、核苷酸使用频率和变异位点统计计算,分析种间核DNA变异,并用芋蝗作为外类群构建NJ和MP分子系统进化树。实验共获得ITS1序列381bp,其中变异位点48个,占所测序列的12.6%,碱基组成分析显示AT含量低于GC含量,与mtDNA富含AT的特征明显不同;核苷酸变异位点统计结果显示,稻蝗属不同种间核苷酸替换数最高为36个,发生在小稻蝗与山稻蝗之间,变异率达11.18%;而无齿稻蝗与短翅稻蝗具有相同的ITS1区序列,彼此之间没有变异。系统发育树显示,小稻蝗和其他稻蝗之间的关系相对较远,是分化较大的种类;日本稻蝗和海南稻蝗与山稻蝗、短翅稻蝗和无齿稻蝗与中华稻蝗关系较近,分别构成一个聚类簇;但是两两种之间的聚类置信度不高,种间关系还有待进一步澄清。The molecular phylogeny of Oxya species were investigated by sequencing the ribosomal DNA （rDNA） ITS1 sequences. We obtained 381bp sequences in length for the seven Oxya species and one out- group species Gesonula punctifrons. The sequences were compared, the nucleotide frequency calculated, and the molecular phylogenetic trees were constructed by NJ and MP methods using G. punctifrons as out- group. The results showed that： （1） the sequence of ITS1 included 48 variable sites （about 12.60%） and showed higher GC content than AT, which was significantly different from mtDNA sequence with much higher AT preference;（2） the largest nucleotide variation was found between O. intricata and O. agavisa with 36 variable sites（about 11.18%）,and no mutation between O. adentata and O. brachyptera; （3） the phylogenetic trees showed that O. hainanensis, O. japonica and O. agavisa was closely clustered; O. brachyptera,O, adentata and O. chinensis have close relationship;O, intricata was obviously remote from other species. In total, the more markers and samples were necessary to further examine the relationship a- mong Oxya species.

关 键 词：稻蝗属 RDNA 序列 系统发育 

分 类 号：Q961[生物学—昆虫学]