远志属腊叶标本DNA提取方法研究  

Study on DNA Extraction from Herbarium Specimens of Genus Polygala

作　　者：樊杰[1] 申飞翔 何益[1] 马全级 箫迪 白妍[1] 束明月 

机构地区：[1]山西中医学院,山西太原030024

出　　处：《山西大学学报（自然科学版）》2014年第3期434-438,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省自然科学基金(2012011034-2);山西中医学院博士科研启动基金(2014);山西中医学院创新团队项目

摘　　要：以远志属植物腊叶标本为例,研究如何从植物腊叶标本中提取到高质量的DNA。在改良CTAB法的基础上,对水浴时间、水浴温度、沉淀时间进行优化。结果表明,降低水浴温度为45℃,延长水浴时间为5-6h,延长沉淀时间为1-2h,可以从远志属腊叶标本中提取到用于PCR反应的DNA。It is difficult to extract high quality DNA from plant spcimens due to DNA degradation and sec- ondary metabolites. As for genus Polygala, how to extrct enough DNA for PCR reaciton is a primary prob- lem. Based on improved CTAB metheod, links of experiment including water bath time and temperature, precipitation time,et al. ,were optimized. The results showed that low temperature water bath at 45 ℃ for a somewhat long time for 5-6 h and a appropriate prolonged precipitation for 1-2 hours can extract DNA for PCR reaction from Polygala specimens.

关 键 词：远志属 腊叶标本 DNA提取 改良CTAB法 

分 类 号：Q946[生物学—植物学]