丙酮酸激酶M1和M2的表达水平与结肠癌发展相关性研究  

Studies on the Correlation between the Expression of Pyruvate Kinase Isoform PKM1,PKM2 and Colon Cancer Development

作　　者：杨鹏[1] 李宗伟[1] 付荣[1] 李汉卿[2] 李卓玉[1] 

机构地区：[1]山西大学生物技术研究所,化学生物学与分子工程教育部重点实验室,山西太原030006 [2]山西大学生命科学学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2014年第3期447-453,共7页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(31271516;31201072);高等学校博士学科点专项科研基金(20111401110011)

摘　　要：研究旨在探讨丙酮酸激酶M1(pyruvate kinase M1,PKM1)、丙酮酸激酶M2(pyruvate kinase M2,PKM2)在不同结肠癌细胞中表达情况与结肠癌发展的相关性。通过Western blot、实时荧光定量PCR、丙酮酸激酶活性分析,检测不同恶性程度结肠癌细胞中PKM1、PKM2的蛋白与基因表达水平及丙酮酸激酶活性,分析它们与结肠癌恶性程度的相关性。PKM1和PKM2在人结肠癌细胞和正常结肠上皮细胞中均有表达;PKM1及PKM2的总蛋白和mRNA表达情况与人结肠癌恶性程度无明显相关性;细胞核中PKM1与PKM2的表达量比值与结肠癌恶性程度呈负相关;丙酮酸激酶活性与结肠癌恶性程度呈正相关。细胞核中PKM1与PKM2的表达量比值可以作为结肠癌恶性评价的一个指标。This study detects the expression of PKM1 and PKM2 in different malignant colon cancer cell lines through detecting gene transcription and protein expression levels. The correlation between the pyru- vate kinases and colon cancer development was studied. Real-time PCR is used to determine the expression levels of gene PKM1 and PKM2. Western blot is applied to detect the expression of PKM1 and PKM2 pro- tein. The pyruvate kinase activity was analyzed in human colorectal cancer cells. The results indicate that there are PKM1 and PKM2 expression in both colon cancer cells and normal colon epithelial cells. Howev- er,there is no clear correlation between either PKM1 and PKM2 mRNA or total protein expression with various stages of colon cancer cells. Notably,the ratio of PKM1 expression and PKM2 expression in cellular nucleus is associated with colon cancer negatively. In addition,Pyruvate kinase activity is positively correla- ted with malignant degree of colon cancer. The ratio of PKM1/PKM2 is correlated with the malignant de- gree of colon cancer strongly and it might be use as a tumor therapeutic index in the future.

关 键 词：PKM1 PKM2 能量代谢 结肠癌 

分 类 号：Q816[生物学—生物工程]