基于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯电化学免疫传感器检测Anti-IgG含量的研究  

Immuno-electrochemical Detection of Anti-IgG Using 3,5-Dibromo Alkali Nickel Complex/Graphite Oxide Composite Modified Electrode

作　　者：赖丽燕[1,2] 刘峥[1,2] 李巍[1,2] 周昱[1,2] 黄红霞[1,2] 

机构地区：[1]桂林理工大学广西矿冶与环境科学实验中心,广西桂林541004 [2]桂林理工大学化学与生物工程学院,广西桂林541004

出　　处：《分析科学学报》2014年第4期481-484,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.21266006);广西自然科学基金(No.2012GXNSFAA053034);广西矿冶与环境科学实验中心资助项目(No.KH2012YB002)

摘　　要：应用物理吸附法将羊抗人IgG抗原直接固定于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯修饰的金电极表面,制备电化学免疫传感器。采用循环伏安法和交流阻抗法对传感器进行表征,结果表明该传感器适合检测Anti-IgG浓度。同时探讨了缓冲液pH值、扫描速度、免疫反应温度、抗原与抗体配比对循环伏安峰电流的影响,结果表明在5-100mV/s扫速范围内,峰电流与扫速呈线性。孵育最优条件为25℃,h-IgG与Anti-IgG配比为1∶1。循环伏安法研究还表明Anti-IgG浓度在0.01-260μg/L范围内,线性关系良好,相关系数r^2=0.993,检出限（S/N=3）为0.006μg/L,据此建立了检测Anti-IgG浓度的新方法。A highly sensitive electrochemical immunosensor was developed for the determination of antiIgG. The sensor was fabricated by moaifying 3,5-dibromo alkali nickel complex corporated with graphite oxide, and the IgG was directly adsorbed on the modified electrode surface. The electrochemical behaviors of the immunesensor were characterized by cyclic voltammetry and AC impedance, and the result indicated that the sensor was suitable for the Anti-IgG dection. Meanwhile, this paper discussed the influence of the scanning rate, buffer solution pH, immune reaction temperature and antigen/antibody ratio on cyclic voltammetric peak current. The result showed that peak current had an linear relationship with the scan rate at the range 0. 005-0.1 mV/s,indicating adsoption controlled process. The optimal incubation condition was 25 ℃, and the IgG/Anti-IgG ratio was 1：1. Cyclic voltammetry studies also showed that when the range of Anti-IgG concentration was between 0.01 and 260 9g/L,the peak current decreased as Anti-IgG concentration increased. （r^2=0. 993）. The detection limit was 0.01 μg/L （S/N=3）.

关 键 词：氧化石墨烯 3 5-二溴水杨醛席夫碱镍配合物 Anti—IgG 电化学免疫传感器 

分 类 号：O657.15[理学—分析化学]