胶束Brij35增敏荧光法测定4-羟基咔唑  

Determination of 4-Hydroxycarbazole by Brij35 Micellar Enhanced Spectrofluorometry

作　　者：张海容[1] 闫晶晶[1] 陈金娥[1] 

机构地区：[1]忻州师范学院生化分析技术研究所,山西忻州034000

出　　处：《分析科学学报》2014年第4期590-592,共3页Journal of Analytical Science

基　　金：忻州师范学院应用化学创新实践基地项目(2013-31);山西省教育厅强校工程项目(J2012092);忻州师范学院科研基金(2011052);山西省忻州市科技研发项目(忻科发No.201231-20)

摘　　要：用荧光光度法研究了水、甲醇、乙醇十二烷基硫酸钠（SDS）、β-环状糊精（β-CD）、溴化十六烷基三甲铵（CTMAB）、聚氧乙烯月桂醚（Brij35）7种不同介质,及反应时间、金属离子、pH条件对4-羟基咔唑荧光性质的影响。结果表明,7种不同介质中Brij35在酸性条件下对4-羟基咔唑荧光增敏效果最好,在λex/λem=337/357nm处有强荧光峰。据此,建立了一种用Brij35增敏荧光光度法测定4-羟基咔唑含量的新方法,其线性范围为4.0×10^-7～1.0×10^-4 mol/L,检出限为6.5×10^-8 mol/L,相对标准偏差（RSD）为2.91%。The effects of reaction time,metal ions and pH on the fluorescence properties of 4-hydroxycarbazole in different media such as water, ethanol, methanol, SDS,β-CD, CTMAB and Brij35 were investigated by spectrofluorometry. The results suggested that the fluorescence intensity of 4-hydroxycarbazole was greatly enhanced in Brij35 solution with a strong peak at λex/λem=337/357nm. A method for the determination of 4-hydroxycarbazole by Brij35 micellar enhanced spectrofluorometry was developed. The linear range of this method was 4.0×10^-7～1.0×10^-4 mol/L with the detection limit of 6.5×10^-8 mol/L and RSD of 2.91%.

关 键 词：4-羟基咔唑 Brij35 胶束增敏荧光光度法 

分 类 号：O657.39[理学—分析化学]