超高效液相色谱-串联质谱法同时测定中成药和保健品中9种抗抑郁药物  

Simultaneous determination of nine antidepressants in traditional Chinese medicines and health food by ultra performance liquid chromatography with tandem mass spectrometry

作　　者：勾新磊[1] 高峡[1,2] 胡光辉[1] 刘伟丽[1,2] 

机构地区：[1]北京市理化分析测试中心,北京100089 [2]国家食品质量安全监督检验中心,北京100094

出　　处：《色谱》2014年第8期822-826,共5页Chinese Journal of Chromatography

基　　金：北京市自然科学基金项目(2122018);北京市科学技术研究院创新团队计划项目(IG200801N/C2)

摘　　要：建立了同时测定中成药和保健品中9种抗抑郁药物的超高效液相色谱-串联质谱（ UPLC-MS / MS）分析方法。以甲醇为提取溶剂，超声提取，离心后取上清液，使用 Waters ACQUITY UPLC BEH C 18色谱柱（100 mm＆#215;2.1 mm，1.7μm）进行分离，以乙腈和0.1％（v / v）甲酸水为流动相对目标物进行梯度洗脱。在电喷雾正离子模式下，采用多反应监测（MRM）模式进行定性和定量分析。结果表明，9种抗抑郁药物在0.04～20μg / L 范围内线性关系良好，线性相关系数（R2）均大于0.998；目标物的检出限为0.0068～0.034μg / L；在0.5、1、5μg / L 3个添加水平下的平均回收率为77.7％～100.8％，相对标准偏差为0.9％～9.1％。本方法准确、简便、快速，可用于中成药和保健品中抗抑郁药物的检测。A sensitive method was developed for the simultaneous determination of nine antide-pressants in traditional Chinese medicines and health food by ultra performance liquid chroma-tography-tandem mass spectrometry（ UPLC-MS / MS）based on the sonication-assisted extrac-tion. The separation was performed on a Waters ACQUITY UPLC BEH C 18 column（100 mm＆#215;2. 1 mm,1. 7 μm）by using 0. 1%（v / v）formic acid aqueous solution and acetonitrile as the mobile phases with gradient elution at a flow rate of 0. 2 mL / min. The electrospray ionization（ ESI） source in positive ion mode was used for the analysis of the nine antidepressants in the multiple reaction monitoring（ MRM ） mode. The standard curves for the nine antidepressants were obtained with good correlation coefficients（ R 2 ﹥0. 998）in the concentration range of 0. 04-20μg / L. The limits of detection for the nine antidepressants were in the range of 0. 006 8- 0. 034μg / L. The average recoveries at three spiked levels（ 0. 5,1,5 μg / L）were 77. 7% - 100. 8%with the relative standard deviation of 0. 9% -9. 1% . The method is accurate,simple,rapid and feasible for the simultaneous determination of the antidepressants in traditional Chinese medi-cines and health food.

关 键 词：超高效液相色谱-串联质谱 抗抑郁药物 中成药 保健品 

分 类 号：O658[理学—分析化学]