腺苷-利多卡因停搏液对在体犬冠状动脉血管内皮细胞的保护作用  

An experimental study on the endothelium protective effect of adenosine-lidocaine cardioplegia on canine coronary artery

作　　者：芦磊[1] 洪毅[1] 陈春玲[1] 郑宏[1] 

机构地区：[1]新疆医科大学第一附属医院麻醉科,乌鲁木齐830054

出　　处：《中华实验外科杂志》2014年第8期1684-1686,共3页Chinese Journal of Experimental Surgery

基　　金：新疆维吾尔自治区教育厅新疆研究生科研创新资助项目（XJGRI2013078）;新疆医科大学第一附属医院青年科研基金资助项目（2012QN06）

摘　　要：目的 观察一氧化氮（NO）、内皮素（ET）和循环内皮细胞（CEC）水平变化以及冠状动脉（简称冠脉）舒缩功能变化,探讨腺苷-利多卡因停搏液对在体犬冠脉血管内皮细胞的保护作用及其机制.方法 12条杂种犬随机分为实验组腺苷-利多卡因停搏液（AL组,n=6）和对照组-ST.thomasⅡ停搏液（K组,n=6）.测定术前（T1）、心脏复跳后1 h（T2）和复跳后2h（T3）NO、ET和CEC水平变化,观察冠脉血管舒张功能变化,并进行电镜下超微结构分析.结果 两组术前基础值差异无统计学意义（P＞0.05）.T3时K组血浆中NO含量较AL组明显减少（P＜0.05）,ET水平明显升高（P＜0.05）,CEC数目明显增多（P＜0.05）.K组冠脉血管舒张最大百分比为（29.13±8.24）％,低于AL组的（68.52±11.32）％ （P ＜0.01）.电镜结果提示,K组冠脉内皮细胞结构损伤较AL组更严重.结论 腺苷-利多卡因停搏液对犬冠脉血管内皮细胞保护效果优于ST.thomasⅡ停搏液.Objective By observing the changes in levels of nitric oxide （NO）,endothelin （ET） and circulating endothelial cells （CEC）,and that in functions of coronary vasomotor,the author discusses the protective effects of adenosine-Lidocaine cardioplegia on endothelial cells of canine coronary artery and its possible mechanism.Methods 12 mongrel dogs were randomly divided into two groups：experimental group with Adenosine-lidocaine cardioplegia （AL group,n =6） and the control group with ST.Thomas Ⅱ cardioplegia （K group,n =6）.Measured the changes in levels of NO,ET and CEC on three time points：before operation （T1）,1 h after heart re-beat （T2） and 2 h after heart re-beat （T3）,observed the changes in functions of coronary vasodilation,and analyzed the coronary ultrastructure by using electron microscope.Results There were no statistical differences between the two groups in T1.On the time point of T3,NO in plasma of group K has been significantly decreased than that of group AL （P ＜ 0.05）.ET has been increased significantly （P ＜ 0.05）,so as CEC （P ＜ 0.05）.The maximum percentage of coronary vasodilation in group K is （29.13 ± 8.24）％ which is lower than that of AL group （68.52 ± 11.32）％ （P ＜ 0.01）.Electron microscopy results show that the damage of coronary vascular endothelial cell ultrastructure of group K is more serious than that of group AL.Conclusion The protective effect of Adenosine-Lidocaine cardioplegia on canine coronary vascular endothelial cell is superior to that of ST.Thomas Ⅱ cardioplegia.

关 键 词：腺苷 利多卡因 体外循环 冠状动脉 循环内皮细胞 

分 类 号：R614[医药卫生—麻醉学]