成骨微环境中低剂量射线对成骨细胞的影响  

Effect of low dose X-irradiation on osteoblastic UMR-106 cells in the osteogenesis microenvironment

作　　者：王勤[1,2] 王新峦[2] 邓晔坤[1] 周晓中[1] 秦岭[2] 

机构地区：[1]苏州大学附属第二医院骨科,215004 [2]中国科学院深圳先进技术研究院转化医学中心

出　　处：《中华实验外科杂志》2014年第8期1775-1777,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（8117712）;江苏省医学重点人才资助项目（2011-15）;江苏省“十二五”临床医学重点学科资助项目

摘　　要：目的 观察成骨诱导微环境中低剂量X射线对成骨细胞的影响.方法 照射后分别检测细胞增殖、碱性磷酸酶（ALP）活性、细胞矿化、成骨特异性转录因子2（Runx2）、骨钙素（0C）的mRNA和蛋白表达.结果 1.0Gy组与对照组比较增殖受到抑制（P ＜0.05）;ALP活性在0.1Gy组[（5.88 ±0.14） nmol对硝基苯磷酸二钠（pNPP）/μg蛋白]与对照组[（4.75±0.05） nmol pNPP/μg蛋白]比较升高（P＜0.05）;矿化定量0.1Gy组（6.57±0.20）与对照组（5.39±0.28）比较升高（P＜0.05）;诱导组Runx2基因0.1 Gy组（5.51±1.52）与对照组（2.43±0.3）比较升高（P＜0.05）,OC基因0.1 Gy组（2.92±1.34）与对照组（1.03±0.41）比较升高（P＜0.05）;诱导组Runx2蛋白表达0.1 Gy组（0.84±0.03）高于对照组（0.44 ±0.02,P＜ 0.05）,OC蛋白表达0.1 Gy组[（427.7±64.2）ng/L]高于对照组[（278.9±53.7） ng/L,P＜0.05].结论 成骨诱导微环境在低剂量射线促进成骨细胞的分化和矿化中起重要呈递作用.Objective To observe the effect of low dose X-irradiation on the proliferation,osteogenic differentiation and mineralization of osteoblastic UMR-106 cells in the osteogenesis microenvironment.Methods After exposure,cell proliferation,osteogenic differentiation and calcium deposition were evaluated by cell counting kit-8 （CCK-8） assay,alkaline phosphatase （ALP） viability,Alizarin Red S staining,respectively.The mRNA and protein levels of Runt-related transcription factor 2 （Runx2） and Osteocalcin （OC） were detected.Results The relative proliferation rate of the cells exposed to 1.0 Gy was significantly lower than that of control （P ＜ 0.05）.The ALP activity of 0.1 Gy group [（5.88 ± 0.14） nmol p-Nitrophenyl Phosphate （pNPP）/μg protein] was significantly increased than that of control group [（4.75 ± 0.05） nmol pNPP/μg protein,P ＜ 0.05].The optical density （OD） value of calcium deposition of 0.1 Gy group （6.57 ± 0.20） was significantly increased than that of control group （5.39 ± 0.28） （P ＜0.05）.In osteogenesis microenvironment groups,the Runx2 mRNA expression of 0.1 Gy group （5.51 ± 1.52） was significantly increased than that of 0 Gy group （2.43 ± 0.3） （P ＜ 0.05）,the OC mRNA expression of 0.1 Gy group （2.92 ± 1.34） was significantly increased than that of 0 Gy group （1.03 ± 0.41） （P ＜ 0.05）.In osteogenesis microenvironment groups,the Runx2 protein expression of 0.1 Gy group （0.84 ± 0.03） was significantly increased than that of 0 Gy group （0.44 ± 0.02,P ＜ 0.05）,the OC protein expression of 0.1 Gy group （427.7 ± 64.2） ng/L was significantly increased than that of 0 Gy group（278.9 ±53.7） ng/L （P＜0.05）.Conclusion The osteogenesis microenvironment plays a present role of low dose X-irradiation promoting osteogenic differentiation and mineralization on osteoblastic cells.

关 键 词：微环境 低剂量射线 成骨特异性转录因子2 骨钙素 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]