不同物种干细胞因子基因编码区生物信息学分析  

Bioinformatics Analysis of Coding Regions of SCF Gene in Different Species

作　　者：锡建中[1] 周荣艳[1] 张军杰[1] 李祥龙[1,2] 李兰会[1] 李楠[1] 张振红[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]河北科技师范学院动物科技学院,河北昌黎066004

出　　处：《中国畜牧兽医》2014年第8期14-20,共7页China Animal Husbandry & Veterinary Medicine

基　　金：河北省自然科学基金项目(C2012204009);河北省教育厅项目(Q2012074)

摘　　要：为了解不同物种干细胞因子(stem cell factor,SCF)基因编码区(CDS)的遗传变异,本研究采用生物信息学方法比较分析了山羊、绵羊、牛、白犀、野猪、虎鲸、马、雪貂、人、鼩鼱、裸鼢鼠、褐家鼠、长尾毛丝鼠、小家鼠和智利八齿鼠SCF基因编码区的遗传多样性,并对该基因的氨基酸序列、跨膜结构域、导肽、信号肽和二级结构特征进行了预测和分析。结果发现,在15个物种55条基因序列中共检测到294个多态位点,生成36种单倍型,SCF基因序列编码区在物种间存在丰富的遗传多样性;理论等电点分布广泛,多数肽链呈酸性,亲水;N端具信号肽、无导肽,可溶型蛋白无跨膜结构域,跨膜型蛋白有1个长23个氨基酸的跨膜结构域;这些蛋白二级结构主要为α-螺旋、无序卷曲、伸展链及β-转角。To study the genetic variation of the SCF gene CDS in different species,the genetic diversity of the CDS of SCF gene sequences from Capra hircus,Ovis aries,Bos taurus,Ceratotherium simum,Sus scrofa,Orcinus orca,Equus caballus,Mustela putorius furo,Homo sapiens,Sorex araneus,Heterocephalus glaber,Rattus norvegicus,Chinchilla lanigera,Mus musculus and Octodon degus were analyzed using the method of bioinformatics.The characteristics of composition of nucleic acid sequences and amino acid sequences,leader peptides,signal peptide,trans-membrane topological structure and the secondary structure were also analyzed,and some of the foci of intense investigation on the function of SCF were reviewed.The results showed that a total of 294 polymorphic sites were detected from 55 sequences of 15 species,from which 36 hapolotypes were sorted.The genetic diversity of the SCF gene CDS was rich among species.The theoretical isoelectric point of the sequences was widespread,most of the peptide chains were acidic,hydrophilic,with the N-terminal signal peptide and without leading peptide.Soluble peptide had no transmembrane domain,while transmembrane type had a 23 amino acid long transmembrane domain.The mainly secondary structures of these proteins were α-helix,disorderly curly,extended chain and β-corner.

关 键 词：物种 干细胞因子基因 生物信息学分析 

分 类 号：Q78[生物学—分子生物学]