福氏志贺菌2型多重PCR检测方法的建立  

Establishment of Multiplex PCR for Detecting of Shigella flexneri Serotype 2

作　　者：朱阵 王婧[1] 张继瑜[1] 魏小娟[1] 周绪正[1] 郭肖[1] 刘翠翠[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所兽药重点开放实验室,甘肃兰州730050

出　　处：《中国畜牧兽医》2014年第8期34-39,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31272603;31101836);国家现代农业肉牛牦牛产业技术体系建设专项(CARS-38)

摘　　要：为构建一种快速、高效、特异性强的检测与鉴定福氏志贺菌的多重PCR方法,本试验以不同血清型志贺菌中的特有基因ipaH、gtrⅡ、gtrⅩ和R002为扩增目标来设计相对应的引物,在同一PCR体系中对福氏志贺菌进行检测。通过一系列探索及对反应条件的优化调整,最终建立了可在多种细菌中快速检测出福氏志贺菌2型(2a和2b)的方法。结果表明,应用多重PCR可快速、高效、准确的对福氏志贺菌进行验证和鉴别,对于预防实验室菌种污染和志贺氏菌病的临床诊断与治疗有着重要意义。In order to build a fast,efficient and specific multiplex PCR method for detection and identification of Shigella flexneri,this study used specific genes ipaH,gtrⅡ,gtrⅩ and R002 in different serotypes of Shigellato design the corresponding primers to amplify the target fragment,and in the same PCR system validated the Shigella flexneri.At the same time through a series of exploration and adjustments to optimize the reaction conditions,we established a method to detect Shigella flexneri type 2a and 2b out of a variety of bacteria quickly.The results suggested that the multiplex PCR could identificate the Shigella flexneri quickly,efficiently and accurately,and had great significance for the prevention of laboratory contamination,clinical diagnosis and treatment of shigellosis.

关 键 词：多重PCR 福氏志贺菌 特有基因 引物 

分 类 号：R378[医药卫生—病原生物学]