ASCL2基因在体细胞克隆牛和自然繁殖牛肺脏中的甲基化分析  

DNA Methylation Status of ASCL2 Gene in Lungs of SCNT Cattle and Naturally Reproduced Cattle

作　　者：王梦楠[1] 张明月[1] 李宁[2] 李世杰[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]中国农业大学农业生物技术国家重点实验室,北京100193

出　　处：《中国畜牧兽医》2014年第8期71-75,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31372312);河北省自然基金项目(C2011204001)

摘　　要：为探讨ASCL2基因在体细胞克隆牛中的重编程状态,本研究通过亚硫酸氢盐测序法(BSP)对体细胞克隆牛和自然繁殖牛肺脏中ASCL2基因启动子区CpG岛上的CpG位点进行了甲基化状态分析。结果显示,体细胞克隆牛的甲基化水平(28.41%)显著高于正常对照组(7.62%)(P<0.05)。推测ASCL2基因的异常甲基化可能是造成体细胞克隆牛新生死亡及肺脏发育异常的原因之一。In an effort to determine the reprogramming of ASCL2 gene in SCNT bovines,the bisulfite sequencing method（BSP）was used to analyze the DNA methylation status in promoter region of ASCL2 gene in lung tissues of SCNT cattle and natural breeding cattle.The results showed that the methylation level of ASCL2 promoter exhibited seriously hypermethylated in SCNT bovines（28.41%）compared with naturally breed bovines（7.62%）（P〈0.05）.Our results suggested that the abnormal DNA methylation of ASCL2 might be associated with the defects of lung organs in died SCNT bovines,which was one of the main reasons for neonatal death of SCNT animals.

关 键 词：ASCL2 重编程 体细胞克隆牛 BSP DNA甲基化 器官缺陷 

分 类 号：Q813[生物学—生物工程]