抗氧氟沙星单克隆抗体的制备及ELISA快速试剂盒的初步研制  

Preparation of Monoclonal Antibodies against Ofloxacin and Preliminary Development of its Enzyme Linked Immunosorbent Assay Kit for Rapid Detection

作　　者：董李学 冯才伟 冯静 郑百芹 贾芳芳 蒙君丽 郝立武 张书宏 

机构地区：[1]唐山市畜牧水产品质量监测中心,河北唐山063000 [2]北京勤邦生物技术有限公司,北京102206

出　　处：《中国畜牧兽医》2014年第8期90-94,共5页China Animal Husbandry & Veterinary Medicine

基　　金：十二五"国家科技支撑计划项目<食品质量安全控制及检测关键技术与产品>之课题"水产品加工过程质量安全控制关键技术集成与示范(2012BAD29B06)

摘　　要：本试验通过碳二亚胺法,将半抗原氧氟沙星与载体牛血清白蛋白（BSA）和卵清白蛋白（OVA）偶联,制备得到氧氟沙星免疫原和包被原,并制备抗氧氟沙星的单克隆抗体,初步研制测定氧氟沙星的ELISA试剂盒,经过测试,对鱼肉样本中氧氟沙星的检测限为1μg/kg,添加回收率在62.8%-97.7%之间,批内、批间试验的相对标准偏差均小于10%。Ofloxacin was coupled with BSA as an immunogen and with OVA as a coating antigen using the coupling agent EDC.Monoclonal antibodies against ofloxacin and its enzyme linked immunosorbent assay kit were prepared and established,respectively.The limit of detection was 1μg/kg in fish,and the recovery range was 62.8% to 97.7%.The intra and inter assay RSD were both less than 10%.

关 键 词：氧氟沙星 单克隆抗体 ELISA试剂盒 快速检测 

分 类 号：S852.43[农业科学—基础兽医学]