锌指核酸酶技术在动物转基因中的研究进展  

Research Advance on Zinc-finger Nucleases Technology in Mammalian Transgenesis

作　　者：袁玉国[1,2] 彭秋玲[3] 

机构地区：[1]扬州大学兽医学院,江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009 [2]扬州大学动物科学与技术学院,江苏扬州225009 [3]宜春学院化学与生物工程学院,江西宜春336000

出　　处：《中国畜牧兽医》2014年第8期188-192,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31101871);江苏高校优势学科建设工程资助项目;中国博士后科学基金资助项目;国家转基因生物新品种培育重大专项(2013ZX08008-004)

摘　　要：锌指核酸酶(zinc-finger nucleases,ZFNs)技术是近年来发展起来的一种基因修饰技术,利用ZFNs可特异识别靶位点DNA序列并使其断裂。与传统基于同源重组的基因打靶相比,ZFNs能使打靶效率提高100000倍,并使基因断裂与突变修复达到同样效果。ZFNs已经应用于多种生物,包括昆虫、两栖类生物、线虫、植物、多种动物和人类细胞。这些应用提高了人们对复杂生理系统的理解,使ZFNs成为一种生产转基因动物、细胞系和植物的有力工具,并在治疗人类疾病中发挥重要作用。文章对ZFNs技术原理及其应用于哺乳类动物转基因的研究进行了回顾和展望。Zinc-finger nucleases（ZFNs）are engineered site-specific DNA cleavage enzymes that may be designed to recognize long target sites and thus cut DNA with high specificity.ZFNs mediate permanent and targeted genetic alteration via induction of a double-strand break at a specific genomic site.Compared to conventional homology-based gene targeting,ZFNs can increase the targeting rate by up to 100000-fold;gene disruption via mutagenic DNA repair is similarly efficient.The utility of ZFNs has been shown in many organisms,including insects,amphibians,plants,nematodes,and several mammals,including humans.This broad range of tractable species renders ZFNs a useful tool for improving the understanding of complex physiological systems,to produce transgenic animals,cell lines,and plants,and to treat human disease.This review summarized recent advances in the understanding of role and function of ZFNs and described the utility of ZFNs for mammalian transgenesis.

关 键 词：锌指核酸酶 动物转基因 基因打靶 

分 类 号：Q78[生物学—分子生物学]