胎盘滋养细胞凋亡在HBV宫内感染中的作用研究  

Effect of JEG-3 apoptosis on HBV intrauterine infection

作　　者：王慰敏[1] 魏小华[1] 白桂芹[1] 付逢萍[1] 袁永兴[1] 李琛[1] 

机构地区：[1]西安交通大学医学院第一附属医院妇产科,陕西西安710061

出　　处：《中国妇幼健康研究》2014年第4期571-573,576,共4页Chinese Journal of Woman and Child Health Research

摘　　要：目的探讨乙型肝炎病毒(HBV)体外感染人绒膜滋养细胞株JEG.3后对细胞凋亡水平的影响,明确HBV感染胎盘的可能机制。方法用人HBVDNA^+血清体外感染JEG-3,免疫组化和Western Blot检测细胞内HBVX抗原(HBxAg)的表达;用Annexin-V荧光素探针标记细胞表面的PS磷脂酰丝氨酸,利用流式细胞仪检测早期细胞凋亡,碘化丙啶(PI)检测中晚期细胞凋亡。结果 HBV体外感染人绒癌细胞株JEG-3后,细胞内检测到HBxAg的表达;细胞的早期凋亡率和晚期凋亡率明显降低。流式细胞仪Annexin V+PI双染方法检测细胞凋亡状态,HBV血清感染组细胞的早期凋亡水平和晚期凋亡水平均低于正常人血清对照组(t值分别为3.27、2.01,均P<0.05),差异均有统计学意义。结论 HBV体外感染人绒癌细胞株JEG-3可表达HBxAg,从而抑制了细胞的早期凋亡及晚期凋亡;其是HBV感染胎盘的可能机制。Objective To explore the influence of JEG-3 infected with hepatitis B virus （HBV） in vitro on apoptosis level, so as to clarify the possible mechanism of HBV infection in placenta. Methods HBV serum infected the human ehorionic trophoblast cell line （ JEG-3 ） in vitro. The intracellular expression of HBV X antigen （HBxAg） was detected by immunohistochemistry and Western Blot, and the PS phosphatidylserine on the cell surface was labeled with Annexin-V fluorescein probe. The early cell apoptosis was detected with flow cytometry and the late cell apoptosis was detected with propidium iodide. Results HBxAg could be found intracellularly after HBV infection in vitro. The early and late cell apoptotic rate reduced compared with the control group which was detected by flow cytometry Annexin V and PI staining methods （ t value was 3.27 and 2.01, respectively, both P 〈 0.05 ）. Conclusion JEG-3 can express HBxAg ＇after HBV infection in vitro, and thus inhibits the cell aDoptosis, which may be the mechanism of HBV infection in placenta.

关 键 词：乙型肝炎病毒 细胞凋亡 乙型肝炎病毒X抗原 滋养细胞 

分 类 号：R512.62[医药卫生—内科学] R321[医药卫生—临床医学]