儿童RRTIs T细胞亚群和NK细胞的变化分析  

Analysis of the variations of T cell subset and NK cells in children with RRTIs

作　　者：席卫平[1] 朱镭[1] 卫重侠[1] 杨建平[1] 任丽丽[1] 

机构地区：[1]山西省妇幼保健院儿童医院,山西太原030013

出　　处：《中国妇幼健康研究》2014年第4期678-680,共3页Chinese Journal of Woman and Child Health Research

基　　金：2009年山西省卫生厅科技攻关计划资助项目(200931)

摘　　要：目的了解3~6岁儿童反复呼吸道感染(RRTIs)T细胞亚群及自然杀伤细胞(NK)的变化,指导临床预防用药,避免过度以及不当治疗。方法严格按照入选标准和剔除标准收集3~6岁儿童160例,其中:病例组73例,对照组87例。采用病例对照研究。用流式细胞仪对病例组和对照组分别进行T细胞亚群、NK细胞的检测,并对有关数据进行统计分析。结果对T细胞亚群(CD3^+、CD4^+、CD8^+、CD4^+/CD8^+)进行两独立样本的Wilcoxon秩和检验,结果表明:CD3^+(z=-1.588,P=0.112)、CD4^+(z=-0.541,P=0.588)、CD8^+(z=-0.733,P=0.463)、CD4^+/CD8^+(z=-0.315,P=0.753)病例组和对照组比较差异均无统计学意义(均P>0.05)。对NK细胞(CD3^+CD16^+CD56^+)进行t'检验(t'=2.779,P=0.006<0.05;95%CI:0.674~4.003),病例组和对照组比较差异有统计学意义。结论对RRTIs儿童检测T细胞亚群与NK细胞具有显著的临床指导意义,可避免过度使用免疫调节剂引起的用药不当。Objective To investigate the variations of T cell subset and NK cells in children aged 3-6 years old with recurrent respiratory tract infections （RRTIs） , so as to provide new guidance for clinical preventive medication and avoid unreasonable therapy. Methods In a case-control study, 160 children aged 3-6 years old were selected strictly according to criterion, including 73 cases in case group and 87 cases in control group. The T cell subset and NK cells from peripheral blood of two groups were measured by flow cytometry method for data analysis. Results Wilcoxon rank-sum test on T cell subset showed that there was no significant difference between two groups in CD3 ＋ （z = -1.588, P=0.112）, CD4 ＋（z= -0.541,P=0.588）, CD8 ＋（z= -0.733, P=0.463） andCD4 ＋/CD8 ＋（z= -0.315, P=0.753） （all P 〉0.05）. But there was significant difference in NK cells between two groups （t＇ = 2. 779, P = 0. 006 〈 0.05; 95% CI： 0. 674- 4. 003 ）. Conclusion Detecting T cell subset and NK cells in children with RRITs is very useful in clinics, which can avoid unreasonable medication caused by excessive immunoregulant.

关 键 词：反复呼吸道感染 病例对照研究 儿童 T细胞亚群 自然杀伤细胞 

分 类 号：R725[医药卫生—儿科]