Hydrogen Sulfide Detection Using a Gold Nanoparticle/Metalloprotein Based Probe  

作　　者：MeisamOmidi 

机构地区：[1]FaeultyofNewScienceandTechnologyUniversityofTehran,Tehran,Iran [2]ResearchCenterforNewTechnologiesinLife-ScienceEngineering,UniversityofTehran,Tehran,Iran [3]SchoolofBiology,CollegeofScience,UniversityofTehran,Tehran,Iran [4]NanobiomedicineCenterofExcellance,UniversityofTehran,Tehran,Iran

出　　处：《Chinese Physics Letters》2014年第8期170-173,共4页中国物理快报（英文版）

摘　　要：We present a simple method for direct detection of hydrogen sulfide （H2S） in an aqueous solution. This method represents a novel biosensor based on metalloprotein cytoehrome c （cyt c） with the localized surface plasmon resonance of gold nanoparticles （AuNPs）. For this purpose, we develop a new approach based on attaching chemically-modified cyt e onto AuNPs. Here, by reacting H2S with protein heine center, its conformation changes in the locality of the heine moiety. The eonformational changes occurring in the protein alter the spectral characteristics by changing the dielectric properties of AuNPs. The conformational changes of eyt c induced by the H2S interaction are characterized by the UV-visible absorption spectroscopy and the circular dichroism technique. The limit of the detection and sensitivity of the AuNPs/cyt c biosensor are evaluated by using UV- visible spectroscopy. According to the experiments, it is revealed that H2S can be detected at a concentration of 4.0μM （1.3ppb） by the fabricated AuNPs/cyt c biosensor. In addition, the sensor retains activity and gives reproducible results after storage in 4℃ for 60 d. This simple and cost-effective sensing platform provides a rapid and convenient detection for H2S at concentrations far below the hazardous limit.We present a simple method for direct detection of hydrogen sulfide （H2S） in an aqueous solution. This method represents a novel biosensor based on metalloprotein cytoehrome c （cyt c） with the localized surface plasmon resonance of gold nanoparticles （AuNPs）. For this purpose, we develop a new approach based on attaching chemically-modified cyt e onto AuNPs. Here, by reacting H2S with protein heine center, its conformation changes in the locality of the heine moiety. The eonformational changes occurring in the protein alter the spectral characteristics by changing the dielectric properties of AuNPs. The conformational changes of eyt c induced by the H2S interaction are characterized by the UV-visible absorption spectroscopy and the circular dichroism technique. The limit of the detection and sensitivity of the AuNPs/cyt c biosensor are evaluated by using UV- visible spectroscopy. According to the experiments, it is revealed that H2S can be detected at a concentration of 4.0μM （1.3ppb） by the fabricated AuNPs/cyt c biosensor. In addition, the sensor retains activity and gives reproducible results after storage in 4℃ for 60 d. This simple and cost-effective sensing platform provides a rapid and convenient detection for H2S at concentrations far below the hazardous limit.

分 类 号：Q556.3[生物学—生物化学] TN379.41[电子电信—物理电子学]