水稻光温敏雄性核不育系扬稻6号S不育基因的遗传分析与分子定位  

Genetic analysis and molecular mapping of a photoperiod-thermo-sensitive genic male sterile gene in rice line Yangdao 6 S

作　　者：潘存红[1] 余玲[1] 戴正元[1] 李爱宏[1] 张小祥[1] 肖宁[1] 李育红[1] 刘广青[1] 王宝和[1] 

机构地区：[1]江苏里下河地区农业科学研究所/国家水稻改良中心南京分中心,江苏扬州225007

出　　处：《扬州大学学报（农业与生命科学版）》2014年第2期48-52,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31201180);水稻生物学国家重点实验室开放课题(110102);江苏省自然科学基金资助项目(BK2011427)

摘　　要：扬稻6号是目前两系杂交籼稻生产中应用面积最大的恢复系，通过“实践八号”育种专用卫星搭载扬稻6号进行航天诱变，筛选出一个光温敏雄性核不育系扬稻6号S。以扬稻6号S与井冈旱稻1号杂交并回交的F2及BC2F2为定位群体进行不育基因的遗传分析。结果表明：扬稻6号S不育性受1对隐性不育基因（暂命名为ptgms2—2）调控，该不育基因被定位于第2染色体标记InDel2-4085和1nDel 2-5846之间，物理距离为200kb。对候选基因进行测序分析，发现在开放阅读框内起始密码子下游70～71bp处存在差异，扬稻6号为GC，扬稻6号S为TA，2个碱基突变导致密码子由GCG变为终止密码子TAG。Yangdao 6 was the most widely used restorer line in the indica two-line hybrid rice breeding system. A pho- toperiod-thermo-sensitive genic male sterile line Yangdao 6 S was selected from the space mutation population of Yangdao 6 via breeding satellite Shijian 8. Genetic analysis indicated that the sterility of Yangdao 6 S was controlled by a single recessive gene using the F2 and BC2F2 population derived from a cross of between Yangdao 6 S and Jingganghandao 1. The sterile gene ptgrns2-2 was restricted to a 200 kb region on chromosome 2 between the marker InDel 2-4085and InDel 2-5846. The sequencing results indicated that there were two nucleotide polymorphisms between Yangdao 6 and Yangdao 6 S. The nucleotide G and C mutated into T and A at site 70-71 bp, subsequently, a premature stop codon was created.

关 键 词：水稻 光温敏雄性核不育性 遗传分析 分子定位 

分 类 号：S511[农业科学—作物学]