电针对POCD模型中老龄大鼠下丘脑-垂体-肾上腺轴及认知功能的影响  

Effect of Electroacupuncture on Middle-aged and Elderly POCD Rats' HPA Axis and Cognitive Function

作　　者：叶雷[1] 刘喆[1] 冯培培[1] 吴萍[1] 潘冰杰[1] 张松[1] 王昕[1] 王文娟[1] 

机构地区：[1]浙江中医药大学第三临床医学院,杭州310053

出　　处：《浙江中医药大学学报》2014年第7期894-899,共6页Journal of Zhejiang Chinese Medical University

基　　金：国家自然科学基金(81273822);973计划课题(2013CB531906);浙江省中医药科学研究基金(2012ZB074)~~

摘　　要：[目的]观察手术创伤应激反应对术后认知功能的影响,探讨电针对术后认知功能障碍(postoperation cognitive dysfunction,POCD)干预效应的下丘脑-垂体-肾上腺轴(Hypothalamic-pituitary-adrenocortical Axis,HPA轴)调节机制。[方法]将100只雌性中老龄SD大鼠随机分为正常组、模型组、电针组和药物组,后三组又分为1d、3d、7d三个亚组,每组10只。采用酶联免疫吸附法测定大鼠血清肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)和皮质酮(CORT)水平,利用morris水迷宫检测电针对大鼠认知功能的改善作用。[结果]POCD模型组动物血清中ACTH、CORT和CRH水平较正常组有显著升高(P<0.05或P<0.01);与模型组相比电针组和药物组血清中ACTH、CORT和CRH水平显著下降(P<0.05或P<0.01),电针组和药物组大鼠在原平台象限停留时间较之模型组均有显著升高(P<0.05或P<0.01)。[结论]手术引起的创伤性应激反应可能是POCD诱发机制之一,电针可通过调节HPA轴对POCD产生治疗作用。[Objective]The purpose of this study is to investigate the effect of electroacupuncture of Baihui and Dazhui points on POCD rats ＇HPA axis and cognitive function. Furthermore, we want to explore the mechanism of electroacupuncture therapy with hypothalamic-pituitary-adrenal axis in POCD.[Methods]100 famale SD rats were randomly divided into control group, model group, electroacupuncture group and drug group. The model,electroacupuncture and drug groups were furtherly divided into 1 day group, 3 days group and 7 days group. The level of ACTH, CORT and CRH in blood serum was measured by enzyme linked immunoadsorbent assay. The improvement of cognitive function in rats was detected by morris water maze.[Results]Compared with control group, the level of ACTH, CORT and CRH in blood serum of POCD model group, P〈0.05 or P〈0.01; Compared with model group, the level of ACTH, CORT and CRH of electroacupuncture and drug group decreased（ P〈0.05 or P〈0.01）. Electroacupuncture and drug group can make the time of rats staying platform quadrant increaseing（P〈0.05 or P〈0.01）.[Conclution]Stress from surgical trauma may be one of inducing mechanisms of POCD.The electroacupuncture can produce a therapeutic effect on POCD by regulating the sthentic HPA axis.

关 键 词：电针 POCD 应激 HPA轴 

分 类 号：R331[医药卫生—人体生理学]