针刺介入时机对缺血性中风大鼠神经功能评分和Bcl-2、Bax表达的影响  

Influence of acupuncture intervention time on neurological function damage of and expression of Bcl-2,Bax protein in rats after ischemic stroke

作　　者：刘勇[1,2] 王洪[3] 赵军[2] 付强[3] 

机构地区：[1]黑龙江中医药大学中西医结合博士后流动站,哈尔滨150040 [2]黑龙江中医药大学附属第一医院针灸一科 [3]黑龙江中医药大学研究生院

出　　处：《环球中医药》2014年第8期581-586,共6页Global Traditional Chinese Medicine

基　　金：黑龙江省博士后资助项目(LBH-Z11011);黑龙江省科技攻关项目(GC08T302)

摘　　要：目的研究不同时间点介入针刺治疗对缺血性中风大鼠神经功能评分和对缺血脑组织中B细胞淋巴瘤/白血病-2(B cell lymphoma/lewkmia-2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)阳性表达的细胞数量的影响,探讨针刺治疗机制及介入时机。方法将200只雄性SD大鼠随机分为假手术组、模型组、2小时针刺组、72小时针刺组和168小时针刺组5组,除假手术组外,均采用线栓法制备大鼠局灶性永久性脑缺血模型,在术后1、3、7、14天,4个时间点采用Garcia复合评分法评价大鼠神经功能,用酶联免疫分析法探查缺血脑组织中的Bcl-2、Bax蛋白表达情况,并在显微镜下记录阳性细胞数目进行比较。结果针刺各组缺血性中风大鼠神经功能评分得到明显改善,优于模型组(P<0.001);2小时针刺组分别优于72小时、168小时组(P<0.01),差异有统计学意义。缺血性中风大鼠术后不同时间点脑组织中的Bcl-2,Bax表达均增高,1天达到高峰,随后下降,与假手术组比较有统计学意义(P<0.001,P<0.01)。术后2小时与模型组、72小时、168小时针刺组比较,可明显提高Bcl-2蛋白的表达、抑制Bax蛋白的表达,差异有统计学意义(P<0.001);72小时与168小时针刺组比较可明显提高Bcl-2蛋白的表达、抑制Bax蛋白的表达,差异有统计学意义(P<0.01)。结论针刺可明显提高缺血性中风大鼠的Garcia评分,改善其运动、感觉和协调功能;针刺可通过调节Bcl-2、Bax蛋白表达来抑制神经细胞凋亡,挽救缺血半暗带神经细胞的功能,而且在缺血性中风后越早介入针刺治疗疗效越好,存在一个最佳治疗时间窗。Objective To study the impact of acupuncture treatment at different time points on neu-rological function score, positive expression cell number of B-cell lymphoma/leukemia-2 （ Bcl-2 ） , Bcl-2 associated X protein （ Bax） in ischemic brain tissue of ischemic stroke in rats, and to investigate the mech-anism and intervention timing of acupuncture treatment. Methods 200 male SD rats were randomly divid-ed into 5 groups：sham operation group, model group, 2 hours acupuncture group, 72 hours acupuncture group and 168 hours acupuncture group. Except the sham group, the rats of all the other groups were pre-pared into permanent focal cerebral ischemia model by suture-occluded method. 1, 3, 7, 14 days after sur-gery, Garcia composite score method was used to evaluate the neural function of the rats at these four time points. Enzyme league immune analysis method was used to probe the Bcl-2 and Bax protein expression in brain tissue, and the number of positive cells under a microscope was recorded for comparison. Results Neurological function score of ischemic stroke rats was significantly improved in acupuncture groups, better than the model group（P〈0. 001）;2 hours acupuncture group was better than that of 72 hours, 168 hours respectively （P〈 0. 01）, and the difference was statistically significant. Bcl-2 and Bax expression in brain tissue at different time points after surgery were increased in ischemic stroke rats, one day reached a peak and then declined, and the difference was statistically significant compared with the model group （ P 〈0. 001, P〈0. 01） . Acupuncture treatment at 2 hours after surgery can significantly increase the expres-sion of Bcl-2 protein, inhibit the expression of Bax protein. , Compared with the model group, 72 hours ac-upuncture group and 168 hours acupuncture group, the difference was statistically significant （P〈0. 001）. Compared with 168 hours acupuncture group, 72 hours acupuncture group can significantly increase the ex-pression of Bcl-2 protein, inhibit

关 键 词：缺血性中风 针刺 神经功能评分 B细胞淋巴瘤 白血病-2 Bcl-2相关X蛋白 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]