MicroRNA-192在UUO幼年大鼠肾间质的表达及雷公藤多苷的干预研究  

Expression of microRNA-192 in kidney interstitium in young rats with unilateral ureteral obstruction and intervention with tripterygium wilfordii polyglycoside

作　　者：刘亚楠[1] 马宏[1] 贾蓉[1] 黄力[1] 

机构地区：[1]山西医科大学第一医院儿科,太原030001

出　　处：《中国药物与临床》2014年第8期1019-1021,共3页Chinese Remedies & Clinics

基　　金：山西高校科技研究开发基金(2003103)

摘　　要：目的：研究幼年大鼠单侧输尿管结扎（UUO）致肾间质纤维化模型中，MicroRNA-192（miR-192）、Ⅰ型胶原的表达以及用免疫抑制剂雷公藤多苷（TWP）的干预效果。方法选用4~5周龄幼年SD雄性大鼠54只，按随机数字表法均分为3组，假手术组、UUO模型组及TWP治疗组，分别于治疗满3、7、14 d杀鼠取左肾，采用实时荧光定量聚合酶链反应（PCR）方法对各组miR-192及Ⅰ型胶原mRNA进行定量检测。结果假手术组miR-192、Ⅰ型胶原mRNA仅微量表达，各时间点组内比较差异无统计学意义（P＞0．05）。与假手术组相比， UUO模型组miR-192、Ⅰ型胶原mRNA表达增加（P＜0．01），且随着梗阻时间的延长，两者的表达逐渐增强，各时间点组内比较差异有统计学意义（P＜0．01）；与UUO模型组相比，TWP治疗组可以减低miR-192、Ⅰ型胶原mR-NA的表达（P＜0．01），但仍高于假手术组（P＜0．01）。结论在幼年大鼠单侧输尿管梗阻致肾间质纤维化模型中， miR-192可能起促进作用；雷公藤多苷可有效抑制miR-192、collagenⅠ的表达，改善肾间质纤维化。Objective To investigate the expression of MicroRNA-192（miR-192）and collagenⅠin kidney in-terstitium in young rats with unilateral ureteral obstruction （UUO）, and to explore the effects of tripterygium wilfordii polyglycoside （TWP）, an immunosuppressive agent on kidney interstitial fibrosis. Methods Fifty-four male SD rats were randomly assigned into sham operation group （n=18）, UUO-model group （n=18） and TWP-treatment group （n=18）. Mice were sacrificed at days 3, 7 and 14 for biopsy of the left kidney. The mRNA of miR-192 and Collagen I were detected by reverse transcription polymerase chain reaction. Results The expression of miR-192 and CollagenⅠmRNA were minimally detectable in the sham-operation group at all time points （both P〉0.05）. Compared with the sham-operation group, the expression of miR-192 and CollagenⅠmRNA were progressively increased with experiment time in the UUO model group （both P〈0.01）. Compared with the UUO group （P〈0.01）, treatment with TWP led to a significant reduction in the expression of miR-192 and CollagenⅠmRNA, despite the higher levels than control group （P〈0.01）. Conclusion miR-192 may play a role in promoting the renal interstitial fibrosis in young rats with UUO. TWP effectively suppresses the synthesis of miR-192 and Collagen I and hinders the progression of renal interstitial fi-brosis.

关 键 词：MicroRNA-192 肾间质纤维化 单侧输尿管结扎 胶原蛋白Ⅰ 雷公藤多苷 

分 类 号：R692[医药卫生—泌尿科学]