硬粒小麦品种Lpx-B1位点等位变异的分子鉴定及其脂肪氧化酶活性  

Molecular Identification of Alleles on Lpx-B1 Locus and Lipoxygenase Activity in Durum Wheat(Triticum turgidum L.)

作　　者：张福彦[1,2] 尚晓丽[1] 吴培培[1] 宋双[1] 陈锋[1] 崔党群[1] 

机构地区：[1]河南农业大学农学院/河南省粮食作物协同创新中心/小麦玉米作物学国家重点实验室,河南郑州450002 [2]河南省核农学重点实验室/河南省科学院同位素研究所有限责任公司,河南郑州450015

出　　处：《作物学报》2014年第8期1364-1370,共7页Acta Agronomica Sinica

基　　金：国家重点基础研究计划(973计划)项目(2014CB138105);教育部新世纪优秀人才项目(NCET-13-0776);河南省高校青年骨干教师资助计划项目(2011GGJS-044);河南省现代农业产业技术体系建设专项(Z2010-01-04)资助

摘　　要：硬粒小麦籽粒中脂肪氧化酶（LOX）与硬粒小麦面制品的加工品质关系密切相关,而 Lpx-B1位点不同变异类型对LOX活性有重要影响。对来自不同国家和地区的167份硬粒小麦品种的LOX活性进行测定,并对其Lpx-B1位点不同变异类型进行分子鉴定。不同品种间LOX活性差异明显,变幅为0.20～7.98 AU min-1 g-1。在Lpx-B1.1位点鉴定出3种等位变异,分别为Lpx-B1.1a、Lpx-B1.1b和Lpx-B1.1c,以Lpx-B1.1a所占比例最高（55.1%）,其次为Lpx-B1.1c （37.1%）,而Lpx-B1.1b仅占7.8%；Lpx-B1.2和Lpx-B1.3二者总是互补出现在不同的品种中,146份品种为Lpx-B1.2型,其余21份品种均为Lpx-B1.3型,表明二者可能互为一对等位因子。在Lpx-B1.1位点的3种等位变异类型中, Lpx-B1.1b类型品种的LOX活性显著高于Lpx-B1.1a和Lpx-B1.1c类型品种,而Lpx-B1.1c类型品种的LOX活性最低。Lpx-B1.3类型品种的LOX活性显著高于Lpx-B1.2类型的品种。参试品种共有6种Lpx-B1基因型组合,其中Lpx-B1.1b/Lpx-B1.3基因型的LOX活性显著高于其他基因型,而Lpx-B1.1c/Lpx-B1.2和Lpx-B1.1c/Lpx-B1.3基因型的LOX活性最低。这些观测结果为硬粒小麦品质育种提供了重要信息。Lipoxygenase （LOX） has a close relationship to the processing quality of flour products in durum wheat （Triticum tur-gidum L.）. Activity of LOX is greatly influenced by the locus of Lpx-B1. The Lpx-B1 alleles in 167 durum wheat varieties from different countries or regions were identified using gene-specific primers, and the Lpx-B1 genotypes were analyzed based on LOX activity data. The LOX activity varied greatly in the 167 varieties, ranging from 0.20 to 7.98 AU min-1 g-1. On Lpx-B1.1 locus, three alleles （Lpx-B1.1a, Lpx-B1.1b, and Lpx-B1.1c） were identified with frequencies of 55.1%, 7.8%, and 37.1%, respectively. Loci Lpx-B1.2 and Lpx-B1.3 were always reciprocally present in varieties, in which Lpx-B1.2 was presented in 146 varieties and Lpx-B1.3 in the remaining 21 varieties. This result indicates the allelic possibility of Lpx-B1.2 and Lpx-B1.3. In the three Lpx-B1.1 genotypes, the Lpx-B1.1b varieties had significantly higher LOX activity than the Lpx-B1.1a and Lpx-B1.1c varieties, and varie-ties carrying Lpx-B1.1c allele possessed the lowest LOX activity. The LOX activity in Lpx-B1.3 genotypes was significantly higher than that in Lpx-B1.2 genotypes. A total of six genotypic combinations were found on the three Lpx-B1 loci in 167 varieties. Among them, Lpx-B1.1b/Lpx-B1.3 combination had the highest LOX activity and combinations of Lpx-B1.1c/Lpx-B1.2 and Lpx-B1.1c/Lpx-B1.3 had the lowest LOX activity. These results are informative to quality breeding of durum wheat.

关 键 词：硬粒小麦 Lpx-B1位点 等位变异 脂肪氧化酶 

分 类 号：S512.1[农业科学—作物学]