萝卜黑斑病菌JZHB_7菌株的生物学特性及培养基的筛选  

The Biological Characteristics of Radish Alternaria and the Screening of the Medium

作　　者：甘彩霞[1] 袁伟玲[1] 王晴芳[1] 崔磊[1] 梅时勇[1] 

机构地区：[1]湖北省农业科学院经济作物研究所,武汉430064

出　　处：《湖北农业科学》2014年第10期2312-2315,共4页Hubei Agricultural Sciences

基　　金：湖北省农业科技创新中心资助项目(2013-620-007-003)

摘　　要：从12个萝卜黑斑病菌（Alternaria sp.）菌株里筛选产孢最多的菌株，从8种培养基（PDA培养基、PSA培养基、牛肉膏蛋白胨培养基、YPD培养基、面粉培养基、豆芽培养基、玉米培养基、杏仁培养基）中筛选其最适合的培养基，研究温度、光照、培养时间对其的影响。结果表明，在12个菌株中JZHB7菌株产孢最多；豆芽培养基为JZHB7产孢的最佳培养基；JZHB7孢子15℃时萌发率高，0℃时萌发率低；JZHB7培养4～7d菌丝生长较快，10～13d生长较慢；日光灯照射培养2h产孢量多，照射培养24h产孢量少。The bacterial strain with the largest sporulation quantity was chosen from the 12 Alternaria sp. bacterial strains and the optimal medium was selected from 8 media including PDA medium, PSA medium, beef extract peptone medium, YPD medium, flour medium, bean sprouts medium, corn medium,almond medium. The influence of temperature, light, treatment time on sporulation of Alternaria sp. were studied. The results showed that JZHB7 Alternaria had the largest sporulation quantity. Bean sprouts medium was the best medium among the eight kinds of media,JZHB7 Alternaria spores germination reached the maximum at 15℃ and the minimum at 0℃. JZHB7 Alternaria strains grew fast from 4 d to 7 d and slow from the 10 d to 13 d. Sporulation quantity of JZHB7 Alternaria increased in 2 h and decreased in 24 h.

关 键 词：萝卜黑斑病菌(Alternaria sp ) JZHB7菌株 生物学特性 产孢量 培养基 

分 类 号：S436.31[农业科学—农业昆虫与害虫防治]