Bacillus subtilis Z-27制剂饲喂断奶仔猪应用研究  

Application of Bacillus subtilis Z-27 Preparation on Feeding Post-weaning Piglets

作　　者：孔少元 姜军坡[1] 周曙光 朱宝成[1] 王世英[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]保定市畜牧工作站,河北保定071000

出　　处：《湖北农业科学》2014年第11期2598-2602,共5页Hubei Agricultural Sciences

基　　金：国家星火计划项目(2010GA105010);河北省石家庄市科学技术研究与发展计划项目(08150142A)

摘　　要：为了研究枯草芽孢杆菌(Bacillus subtilis)Z-27制剂(以下简称Z-27菌剂)对断奶仔猪肠道中微生物菌群及消化酶活力、生长性能和腹泻率的影响,将供试仔猪分为试验组和对照组,试验组饲喂添加0.1%Z-27菌剂的T2饲料,对照组饲喂T2饲料;采集粪便并使用鉴别培养基进行培养和菌落计数;测定仔猪粪便中蛋白酶、α-淀粉酶、脂肪酶和纤维素酶等消化酶活力;记录并计算各组仔猪的平均日增重、料重比及腹泻率。结果表明,与对照组相比,试验组仔猪粪便中大肠杆菌(E.coli)数量降低了37.6%,乳酸菌(Lactobacillus)、双歧杆菌(Bifidobacterium)、芽孢杆菌数量分别增加了45.7%、47.4%、40.1%;试验组粪便中蛋白酶、α-淀粉酶、脂肪酶和纤维素酶的平均酶活力分别增加了62.99%、34.56%、66.57%和133.10%;平均日增重增加了9.37%,料重比降低了4.94%,腹泻率下降83.22%。表明枯草芽孢杆菌Z-27制剂能够抑制大肠杆菌等肠道病原菌,促进乳酸菌等有益菌的生长,维护仔猪肠道微生态平衡,有效预防仔猪腹泻,同时产生多种外源消化酶,有利于提高饲料转化率,提高仔猪生长性能。The effects of Bacillus subtilis Z-27 preparation on microflora and digestive enzymes in piglets intestinal,growth performance and diarrhea rate were studied through the comparative test of two groups fed adding with 0.1% Z-27 preparation in T2 feed and only T2 feed separately.Colonies in piglets＇ fecal samples were cultured and counted with differential medium.Digestive enzymes activities such as protease,α-amylase,lipase and cellulase in piglets＇ fecal samples and the average daily gain,feed/gain ratio and diarrhea rate were measured and recorded.The results showed that compared with the control group the E.coli in fecal samples of the test group was decreased by 37.6%,while Lactobacillus,Bifidobacterium and Bacillus was increased by 45.7%,47.4% and 40.1%,respectively.Protease,α-amylase,lipase activity and cellulase in fecal samples of the test group was increased by an average of 62.99%,34.56%,66.57% and 133.10%,respectively.Average daily gain of piglets was increased 9.37% while feed/gain ratio and diarrhea was decreased 4.94% and 83.22%,respectively.It is indicated that Bacillus subtilis Z-27 preparation could inhibit E.coli and promote the growth of beneficial bacterium,which was in favor of maintaining microecological balance and effectively preventing diarrhea.The exogenous digestive enzymes produced by Z-27 preparation could enhance feed efficiency and improve the performance of post-weaning piglet.

关 键 词：枯草芽孢杆菌(Bacillus subtilis)Z-27制剂 断奶仔猪 肠道菌群 消化酶 腹泻 

分 类 号：S828.5[农业科学—畜牧学] S816.79[农业科学—畜牧兽医]