响应面分析法对大火草多糖提取工艺的优化  

Optimizing the Process of Extracting Polysaccharides from Anemoen tomentose (Maxim.) Péi by Response Surface Methodology

作　　者：贝盏临[1] 张欣[1] 曹君迈[1] 雷茜[1] 谢杏[1] 

机构地区：[1]北方民族大学生物科学与工程学院,银川750021

出　　处：《湖北农业科学》2014年第11期2616-2620,共5页Hubei Agricultural Sciences

基　　金：国家民委生态系统模型及应用重点实验室项目(2010SY08)

摘　　要：采用超声波和微波技术提取大火草[Anemone tomentosa(Maxim.)Péi]根、茎、叶中的多糖,确定超声波醇提为最佳提取方法,用苯酚-硫酸法测定其多糖含量。通过单因素试验和响应面分析法,研究了料液比、温度、提取时间对多糖提取率的影响。结果表明,提取时间和温度是影响大火草多糖提取率的主要因素,试验稳定性、精密度良好,提取大火草多糖的最佳条件为温度80℃,提取时间50 min,料液比1∶100(m∶V)。利用最佳提取工艺得到根、茎、叶中的多糖含量分别为5.16%、1.61%、7.16%。The polysaccharides were extracted from the roots,stems and leaves of Anemone tomentosa（Maxim.） Péi with ultrasonic and microwave extraction.The ultrasound-assisted extraction with ethanol was the best method.Polysaccharides were measured by phenol-sulfuric acid method.The effects of material-liquid ratio,extraction temperature and time on the extraction of polysaccharides were studied with single-factor experiment and response surface methodology.The results showed that the temperature and the time were the main factors.The optimal condition of polysaccharides extraction was temperature of 80℃,extraction time of 50 min,material-liquid ratio of 1∶100（m∶V）.Under the optimal conditions,the contents of polysaccharides in roots,stems and the leaves were 5.16%,1.61% and 7.16%,respectively.

关 键 词：大火草[Anemone tomentosa(Maxim )Péi] 多糖 响应面分析法 

分 类 号：Q949.95[生物学—植物学]