多种方法提取苎麻组织基因组DNA的质量比较  

Comparison of the Genomic DNA Extraction Methods From Different Ramie Tissues

作　　者：张平[1] 夏东升[1] 蔡亚君[1] 曾庆福[1] 王军[1] 

机构地区：[1]武汉纺织大学纺织印染清洁生产教育部工程研究中心,武汉430073

出　　处：《湖北农业科学》2014年第11期2682-2685,共4页Hubei Agricultural Sciences

基　　金：湖北省教育厅优秀中青年科技人才项目(Q20081704);武汉科技学院青年基金项目(20073204);中国纺织工业协会项目(2006074);国家科技支撑计划项目(2010BA02B00);苎麻生态高值利用技术研究与产品开发项目(2012BAD36B03-04)

摘　　要：以苎麻[Boehmeria nivea(L.)Gaudich.]的纤维成熟期韧皮组织、种子和根组织为试验材料,采用CTAB法、SDS法和尿素法提取苎麻基因组DNA。3种DNA提取方法比较,CTAB法提取的DNA质量优于其他两种方法;3种试验材料比较,韧皮组织提取的DNA质量最好,其次是种子和根组织。种子提取的DNA浓度最高。设计梯度试验分析不同研磨时间对韧皮组织和种子总DNA提取质量的影响,结果显示韧皮组织研磨4 min、种子研磨6 min提取的总DNA质量较好。Abatract： Extraction of high quality genomic DNA from Ramie tissue is the foundation for the study of molecular biology of ramie.In order to study ramie genome by some molecular test,such as PCR,In this study,CTAB,SDS and urea methods were used to extract the ramie genomic DNA from the phloem tissue of ramie fiber mature phase,seed and root tissue respectively.The DNA extraction effect of three different tissues and methods were compared by ultraviolet spectrophotometry and agarose electroforesis.The results showed that the extraction effect of CTAB method is best than the other two method;the extraction effect of the phloem tissue of ramie fiber mature phase is best in three different tissues,follow by the seed and root tissue.The seed is able to extract high quantity genomic DNA in three extraction methods.In addition we study different milling time of the phloem tissue and seeds to total DNA quality effect by used CTAB and SDS methods.The results showed that the phloem tissue grinding for 4min,seed tissue grinding for 6 minutes can get high quality DNA.

关 键 词：苎麻[Boehmeria nivea(L )Gaudich ] 基因组DNA 提取 组织 CTAB法 SDS法 尿素法 

分 类 号：R281.4[医药卫生—中药学] Q7[医药卫生—中医学]